枯草芽胞杆菌芽胞表面展示研究进展

Recent Developments in Spore Surface Display of Bacillus subtilis

枯草芽胞杆菌芽胞表面展示技术是把枯草芽胞杆菌作为芽胞表面展示的宿主来展示目的蛋白的一种技术。该技术不仅具备芽胞表面展示技术可展示分子量较大的目的蛋白、目的蛋白无需跨膜及芽胞的极强抗逆性等特点外,同时由于该技术的宿主菌——枯草芽胞杆菌的分子生物学信息研究得比较清楚、安全性高而被广泛应用。介绍了枯草芽胞杆菌表面展示近10年在生产疫苗和固定化酶方面的进展,并对如何提高表面展示目的蛋白的产量做了简要概述。

Bacillus subtilis spore display,a technique that presents proteins or peptides on the surface of the spore of Bacillus subtilis,enables high-throughput screening and has become an essential tool in bimolecular engineering. The major advantage of spores over the other published systems is their synthesis within the cytoplasm of the bacterial cell. Therefore,any heterologous protein to be anchored on the outside does not have to cross any membrane. Furthermore,spores are extremely resistant against high temperature,irradiation and many chemicals,and can be stored for many years at room temperature. With the advantage of complete genome information and security,Bacillus subtilis spore display now is attracting more and more attention. The following describes the recent progress of surface of Bacillus subtilis in the produce of vaccines and the immobillzed enzyme,and present a brief overview about how to improve the yield of the target protein surface display.