鼻型结外NK/T细胞淋巴瘤患者外周血CD4^＋CD25^＋调节性T细胞检测的临床意义

Clinical significance of peripheral blood CD4^＋CD25^＋ regulatory T cell in patients with extranodal NK/T cell lymphoma, nasal type

目的 通过检测鼻型结外NK/T细胞淋巴瘤（ENKL）患者外周血调节性T细胞（Treg细胞）,探讨外周血Treg细胞与ENKL的关系.方法 选取北京同仁医院血液科2009年3月至2012年12月收治的41例初治ENKL患者,20名健康志愿者作为正常对照.采用流式细胞仪联合标记检测外周血Treg（CD4＋CD25＋）细胞,并与淋巴瘤危险因素及疗效、预后等进行相关性分析.结果 ENKL患者治疗前外周血Treg细胞比例为（9.64±4.96）％,高于正常对照组的（7.31±3.02）％,差异具有统计学意义（P＜0.05）;治疗后患者外周血Treg细胞降至（5.18±2.19）％,与治疗前比较差异具有统计学意义（P＜0.01）;Treg细胞比例在治疗有效组（35例）和治疗无效组（6例）分别为（8.79±4.15）％和（14.57±6.73）％,两者差异具有统计学意义（P＜ 0.05）.ENKL患者Treg细胞比例与血清LDH水平呈正相关;Treg细胞比例正常组患者的总生存率和无进展生存率高于Treg细胞比例增高组,但差异无统计学意义.结论 ENKL患者外周血Treg细胞与疗效及血清LDH水平相关,可能对ENKL的疗效及预后有一定的预测作用.

Objective To investigate the clinical significance of peripheral blood regulatory T cell （Treg） population in patients with extranodal NK/T cell lymphoma,nasal type （ENKL）.Methods The peripheral blood CD4＋CD25＋ Treg cell population was detected by flow cytometry in 41 newly diagnosed ENKL patients between March 2009 and December 2012.Results The proportion of CD4＋CD25＋ Treg cell population increased in ENKL patients compared to healthy donors [（9.64±4.96） ％ vs （7.31 ± 3.02） ％,P＜0.05],and decreased significantly after treatment [（5.18 ±2.19） ％,P＜0.01].Patients when got response had significantly lower proportion of Treg cells [（8.79±4.15）％] as compared with those without response [（14.57±6.73）％,P＜0.05].The proportion of Treg population was positively related to the serum lactate dehydrogenass level.Conclusions The proportion of peripheral blood Treg cells may be helpful for predicting prognosis and therapeutic efficacy in ENKL patients.