银耳提取物对酒精性肝损伤辅助保护作用

Auxiliary protective effects of the extracts from Tremella fuciformis fruiting body on alcohol-induced liver injuries in mice

探讨银耳提取物对小鼠肝损伤的保护作用。采用50%乙醇经口灌胃造成小鼠急性酒精性肝损伤模型,将动物随机分成5组,分别是空白对照组,模型组,银耳提取物低、中、高剂量组(3个剂量组分别为225、450、1 350mg/kg BW),检测肝组织中丙二醛(MDA)、还原型谷胱甘肽(GSH)、甘油三酯(TG)的含量,并取肝脏作病理切片,观察肝脏的脂肪病变情况。结果显示银耳提取物3个剂量组肝组织MDA含量均低于模型组(P<0.01);与模型组相比,各组GSH含量虽有所升高,但均无统计学差异(P>0.05);中、高剂量组TG含量低于模型组(P<0.01),且肝细胞脂肪变性均比模型组明显减轻(P<0.01)。结果提示银耳提取物对酒精性肝损伤有辅助保护功能。

The protective effects of extracts from Tremella fuciformis fruiting body on alcohol-induced liver injuries in mice were determined. Fifty mice were randomly divided into five groups： blank control group, model group and prevention groups treated with low dose （225mg/kg BW）, medium dose （450mg/kg BW） and high dose （1 350mg/kg BW） of Tremellafuciformis fruiting body extracts. Except the blank control group, the other groups were liver-injured by 50% alcohol. The contents of malondialdehyde （MDA）, glutathione （GSH） and triglyceride （TG） in liver tissue were determined, and the liver damage was evaluated by histological examination. Compared with the model group, MDA level in liver tissue of the three treatment groups remarkably decreased （P〈0.01）, while the enhancement of GSH level had no statistical difference （P〉0.05）. The TG content and hepatocyte fatty degeneration of the moderate and high dose group were obviously lower than those of the model group. The result suggested that extracts of Tremella fuciformis fruiting body have an auxiliary protective effects on liver injury induced by alcohol in mice