大鼠颈内动脉泵注丙泊酚对脑灌注区血管、神经组织的影响

Effect of internal carotid artery infusion of propofol on neurovascular tissues of cerebral infusion areas in rats

目的 观察丙泊酚经大鼠颈内动脉泵注对脑灌注区皮层组织的影响,探讨丙泊酚颈内动脉麻醉的安全性. 方法 SD大鼠40只按随机数字表法分为右股静脉给药组和右颈内动脉给药组（每组各20只）,麻醉诱导后分别通过右股静脉或右颈内动脉留置针泵注丙泊酚30 mg/（kg·h）,持续3h.停药后4h处死大鼠并取右侧大脑皮层,HE染色、透视电镜观察大鼠右侧大脑皮层组织的形态学和超微结构改变;实时PCR（RT-PCR）检测皮层B细胞淋巴瘤/白血病-2（bcl-2）、bcl-2相关X蛋白（Bax） mRNA的表达. 结果 右股静脉给药组与右颈内动脉给药组大鼠皮层神经元、神经胶质细胞、血管内膜结构均正常,未见急性损伤现象;电镜显示大鼠皮层的神经元细胞核、细胞器、毛细血管内膜结构均正常,未见明显变化;RT-PCR结果显示2组大鼠皮层bcl-2、Bax mRNA的表达比较差异均无统计学意义（P＞0.05）. 结论 丙泊酚经大鼠颈内动脉泵注对脑灌注区皮层组织的影响不显著.

Objective To explore the security of propofol used in internal carotid artery anesthesia by observing the effect of internal carotid artery infusion of propofol on neurovascular tissue of cerebral infusion areas in rats.Methods A total of 40 SD rats were randomly divided into a V group and an A group （n=20）; rats in the V group were given treatment of right femoral vein infusion of 30 mg/ （kg· h） propofol,and those in the A group were given treatment of right internal carotid artery infusion of 30 mg/（kg· h） propofol for 3 h.Rats were sacrificed 4 h after propofol withdrawal.HE staining and transmission electron microscopy were employed to detect the morphological and ultrastructural changes in the right cerebral cortex tissues; and semi-quantitative real time PCR was used to observe the mRNA expressions of bcl-2 and Bax.Results No obvious acute injury was noted in both groups,and the morphology of cortical neurons,neuralgia cells and vascular structures was normal in both groups.And the neuronal nuclei,organelles and capillary endomembrane of both groups were normal,without obvious changes.No significant difference in mRNA expressions ofbcl-2 and Bax was noted between the two groups （P〉0.05）.Conclusion It is insignificant in the effects of internal carotid artery infusion of propofol on neurovascular tissues of cerebral infusion areas in rats.