肝脾调补方对大鼠急性酒精性肝损伤的保护作用

Protection of Ganpitiaobu Fang,a Traditional Chinese Medicine,Against Alcoholic Induced Liver Injury in Rats

目的:观察中药肝脾调补方对大鼠急性酒精性肝损伤的保护作用机制。方法:采用白酒灌胃法建立大鼠急性酒精性肝损伤模型。通过肝脾调补方高、中、低剂量灌胃,观察肝脾调补方对各组大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)及肝组织匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮(NO)水平的影响。结果:肝脾调补方可明显降低ALT、AST含量,显著降低TNF-α、IL-6水平;降低肝组织匀浆的NO的含量,提高肝匀浆SOD、GSH-Px活性。结论:肝脾调补方对急性酒精性肝损伤有保护作用,能改善肝功能,其机制可能与减轻肝脏炎症、抗脂质过氧化有关。

Objective：To study the effect of GanpitiaobuFang,a traditional Chinese medicine,for liver diseases,on alcoholic injury in rats and its pharmacological mechanisms.Methods：Acute hepatic injury model was made by alcohol gavage.Sixty mice were divided into control group,model group,low dose group,middle dose group,and high dose group randomly.The levels of alanine aminotransferase（ALT）and aspartate aminotransferase（AST）,TNF-αand IL-6in plasma,as well as superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）,and nitric oxide（NO）in liver homogenate of the five groups were assayed and compared.Results：Ganpitiaobu Fangreduced serum ALT/AST levels and liver NO/TNF-α/IL-6levels significantly,and elevated liver SOD and GSH-Px levels.Conclusion：Ganpitiaobu Fang can obviously protect rat liver against alcoholic injury maybe through anti-lipid peroxidation and stabilization of liver cell membrane andmitochondrial membrane.