应用基因芯片筛选七氟醚和丙泊酚的诱导下胃黏膜表达差异基因

Identification of sevoflurane and propofol induced differentially expressed genes in gastric mucosa with DNA microarray

目的:本文对行无痛胃镜检查的患者胃黏膜组织芯片表达谱进行分析,分别利用吸入性麻醉剂七氟醚和静脉麻醉剂丙泊酚麻醉,比较分析两种不同种类的麻醉剂诱导下表达发生显著改变的基因,从功能方向筛选麻醉剂保护胃黏膜细胞的分子靶标以及保护机制.方法:从基因表达数据库(Gene ExpressionOmnibus,GEO)数据库中下载编号为GSE4386的基因芯片,数据为无痛胃镜检查的患者胃黏膜组织芯片表达谱数据,共40个样本,平均分成了两个组:吸入性七氟醚麻醉和静脉麻醉剂丙泊酚,每组20个样本,分别为手术麻醉剂处理之前和经过麻醉剂手术后的胃黏膜组织表达谱.采用R软件包对表达谱做数据预处理和差异表达基因的筛选.筛选到的差异基因将进一步生物信息分析.首先比较两个组中筛选得到的麻醉剂处理前后的差异表达基因,得到两种麻醉剂刺激下诱导表达变化的基因,并做各组间的表达值比较;然后通过BLASTX对差异表达基因产物蛋白进行比对后进行COG功能分类注释;再用WebGestalt寻找差异表达基因所在的相互作用功能模块;最后利用DAVID搜索模块中差异基因参与的生物学过程.结果:共同表达的差异基因在七氟醚组和异丙酚组中分别占差异基因数目的百分比为31.3%(275/879)和94.8%(275/290).且从功能注释分类及同在一个模块中差异基因的功能结果,两种麻醉剂诱导影响的表达变异基因倾向于一致.共同表达差异基因,异丙酚诱导的差异基因和七氟醚诱导的差异基因主要参与的细胞转录和分化的过程.结论:吸入性麻醉剂七氟醚和静脉麻醉剂丙泊酚在保护胃黏膜作用中具有协同的作用,因此,我们推测,吸入性麻醉剂七氟醚和静脉麻醉剂丙泊酚两种方式的静吸复合麻醉方式,在无痛胃镜检查术中可能会具有更好保护胃黏膜细胞作用.

AIM： To identify differentially expressed genes （DEGs） induced by sevoflurane and propofol with DNA microarray. METHODS： The expression data of GSE4386 which contained atrial samples collected from patients receiving anesthetic gas sevoflurane or intravenous anesthetic propofol in gastrointestinal endoscopy were downloaded from Gene Expression Omnibus （GEO）. The DEGs in the sevoflurane group and propofol group were identified and compared. Then, the functions significantly related to the DEGs were enriched.The interactive functional modules for common, sevoflurane specific and propofol specific DEGs were constructed to perform analysis of biologi- cal processes. RESU LTS： The percentages of DEGs were 31.3% （275/879） and 94.8% （275/290） in the sevoflu- rane group and propofol group, respectively. Functional categories for the common, sevoflu- rane specific and propofol specific DEGs were very similar. Function modules, such as regula- tion of transcription and regulation of cellular process, for the common, propofol specific and sevoflurane specific DEGs were identifiedl CONCLUSION： Sevoflurane and propofol may synergistically reduce gastric mucosal tissue injury in patients undergoing gastrointestinal endoscopy.