使用GEO数据集分析PFKFB3在肝细胞癌中的表达及临床意义

Analysis of clinical significance of expression of 6-phosphofructo-2-kinase in hepatocellular carcinoma utilizing GEO datasets

目的:通过基因表达汇编(Gene Expression Omnibus,GEO)公共数据库,探讨6-磷酸果糖激酶2(6-phosphofructo-2-kinase,PFKFB3)在肝细胞癌中的表达情况,并进一步探索其与肝细胞癌临床病理特征的关系,评价PFKFB3对肝细胞癌术后患者预后评价的意义,预测PFKFB3推动肝细胞癌发展的机制.方法:检索、下载美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)的肿瘤公共数据集,对表达谱资料及临床信息进行分析;利用基因集富集分析(gene set enrichment analysis,GSEA)方法,分析受PFKFB3调控的相关基因.结果:PFKFB3在高T分期肿瘤中高表达(P<0.05);在不同年龄、谷丙转氨酶(alaninet r a n s a m i n a s e,A LT)、肿瘤大小、肿瘤结节数目、T分期、肝细胞癌巴塞罗那分期(Barcelona Clinic Liver Cancer stage,BCLCstage)、意大利肝细胞癌项目分期(CancerLiver Italian Program stage,CLIP stage)的肝细胞癌患者中,PFKFB3的表达均有显著性差异(P<0.05);PFKFB3的表达状态与肝细胞癌患者术后的总体生存和复发相关(P<0.05);PFKFB3高表达样本富集了与细胞增殖、抗凋亡、代谢相关的基因集.结论:PFKFB3与肝细胞癌多个病理指标相关,且可以做为潜在的判断肝细胞癌患者预后的标志物和治疗肿瘤的靶标.

AIM： To investigate the expression of 6-phos-phofructo-2-kinase（PFKFB3） in multiple tumors,to clarify the relationship between PFKFB3 ex-pression and clinicopathological characteristicsof hepatocellular carcinoma, and to evaluatethe possibility of using PFKFB3 as a prognosismarker in hepatocellular carcinoma. METHODS： GEO datasets were collected andexpression profile and clinical information wereanalyzed. Gene set enrichment analysis（GSEA）was conducted to explore the gene sets enrichedin samples with high PFKFB3 expression. RESULTS： The expression of PFKFB3 wasup-regulated in high grade tumors（P 0.05）. PFKFB3 expression was significantly associated with age, alanine transaminase（ALT）, tumor volume, the number of nodules, T stage, Barcelo-na Clinic Liver Cancer（BCLC） stage and Cancer Liver Italian Program（CLIP） stage. Higher ex-pression of PFKFB3 indicated poor prognosis in hepatocellular carcinoma. GSEA indicated that PFKFB3 regulates gene sets associated with anti-apoptosis, negative regulation of programmed cell death, and positive regulation of cell prolif-eration and cellular metabolic process. CONCLUSION： PFKFB3 is highly expressed in multiple tumors and functions as a potential marker and target for diagnosis and treatment of hepatocellular carcinoma.