氧化应激状态评价对消化系肿瘤诊断的临床指导意义

Clinical significance of oxidative stress evaluation in gastrointestinal cancer diagnosis

目的:对胃肠道肿瘤患者机体氧化应激状态展开分析研究.方法:随机选取2011-09/2013-09青海大学附属医院接收救治的200例胃肠道肿瘤患者,分成胃癌组100例,肠癌组100例,并选取同期于青海大学附属医院进行健康体检正常人50例作为对照组,所有研究对象均检测血清中氧化损伤产物、抗氧化剂及抗氧化酶类.结果:胃癌组和肠癌组蛋白羰基(protein c a r b o n y l)、晚期氧化蛋白产物(a d v a n c e d products)、丙二醛(malondialdehyde)以及8-羟基脱氧鸟苷(8-hydroxy-2'-deoxyguanosine)浓度与健康对照组相比,差异具有统计学意义(4.12 nmol/L±1.34 nmol/L,4.24 nmol/L±1.29 nmol/L vs 2.83 nmol/L±0.71 nmol/L;31.93μmol/L±12.34μmol/L,32.14μmol/L±11.69μmol/L vs 25.91μmol/L±9.10μmol/L;2.98 nmol/L±1.13 nmol/L,3.02 nmol/L±1.24 nmol/L vs 6.13 nmol/L±1.82 nmol/L,125.91 mg/L±5.00 mg/L,124.13 mg/L±5.00 mg/L vs 96.16 mg/L±68.29 mg/L,均P<0.05);胃癌组与肠癌组组间氧化损伤产物浓度对比,差异无统计学意义(均P>0.05);胃癌组和肠癌组谷胱甘肽(glutathione,GSH)、维生素C以及维生素E浓度与健康对照组相比,差异具有统计学意义(均P<0.05);胃癌组与肠癌组组间抗氧化剂浓度对比,差异无统计学意义(均P>0.05);胃癌组与肠癌组超氧化物歧化酶(orgotein superoxide dismutase)、谷胱甘肽过氧化物酶(glutathione peroxidase)以及过氧化氢酶(catalase)活性与健康对照组相比,差异具有统计学意义(均P<0.05);胃癌组、肠癌组组间抗氧化酶类活性对比,差异无统计学意义(均P>0.05).结论:胃肠道肿瘤患者机体受氧化应激影响,评价胃肠道肿瘤患者机体氧化应激状态对判定胃肠道肿瘤发生具有十分重要的临床指导意义.

AIM： To analyze oxidative stress in patients with gastrointestinal tumors. METHODS： Two hundred patients with gastro-intestinal cancer treated from September 2011 to September 2013 at our hospital were randomly selected and divided into a gastric cancer group（n = 100） and a colorectal cancer group（n = 100）. Fifty healthy volunteers receiving physical examination during the same period at our hos-pital were used as controls. All study subjects were subjected to measurement of serum oxida-tive damage products, antioxidants and antioxi-dant enzymes.RESULTS： The concentrations of protein car-bonyl（PC）, advanced oxidation protein products（AOPP）, malondialdehyde（MDA）, and 8-hy-droxy-2＇-deoxyguanosine（8-OHdG） in patients with gastric cancer and colorectal cancer were significantly different from those in healthy con-trols（4.12 nmol/L ± 1.34 nmol/L, 4.24 nmol/L ± 1.29 nmol/L vs 2.83 nmol/L ± 0.71 nmol/L; 31.93 μmol/L ± 12.34 μmol/L, 32.14 μmol/L ± 11.69 μmol/L vs 25.91 μmol/L ± 9.10 μmol/L; 2.98 nmol/L ± 1.13 nmol/L, 3.02 nmol/L ± 1.24 nmol/L vs 6.13 nmol/L ± 1.82 nmol/L; 125.91 mg/L ± 5.00 mg/L, 124.13 mg/L ± 5.00 mg/L vs 96.16 mg/L ± 68.29 mg/L, P 〈0.05）, although there were no significant differences between the gastric cancer and colorectal cancer groups（P 〈0.05）. The concentrations of glutathione, glutathione（GSH）, vitamin C and vitamin E in patients with gastric cancer and colorectal cancer were significantly different from those in healthy controls（P 〈0.05）, although there were no sig-nificant differences between the gastric cancer and colorectal cancer groups（P 〈0.05）. Superox-ide dismutase（SOD）, glutathione peroxide mat-ter enzyme（GSH-Px） and catalane（CAT） activ-ity in patients with gastric cancer and colorectal cancer were significantly different from those in healthy controls（P 〈0.05）, although there were no significant differences between the gastric cancer and colorectal cancer groups（P 〈0.05）.CONCLUSION： Patients with gastrointestinal tu-mors undergo oxidative stress, and evaluation of oxidative stress in patients with gastrointestinal cancer has important clinical significance in deter-mining the occurrence of gastrointestinal cancer.