激光光凝法建立小鼠慢性高眼压模型

Mice mode of high intraocular pressure established by laser photocoagulation

目的：激光光凝法建立小鼠的慢性高眼压模型,为以后的研究做准备。方法：C57BL/6小鼠44只一侧眼用来诱导慢性高眼压。另一只眼作为对照眼。TONO-PENAVIA眼压笔分别在激光光凝后1,2,4,8wk测量小鼠眼内压。裂隙灯显微镜观察小鼠眼球结构变化情况,分别于术后1,2,4,8wk摘除小鼠双侧眼球,进行冰冻切片,采用HE染色,光镜下观察视网膜情况。TUNEL计数视网膜切片的细胞凋亡。结果：在1~8wk,实验眼的眼内压明显高于对照眼（P〈0.05）,最高眼内压是31mmHg,只有1眼。眼压平均在20mmHg左右。在第2wk,内核层和视网膜神经节细胞层细胞数较对照眼轻度减少,在第4,8wk细胞数明显减少。结论：角膜缘激光光凝能造成小鼠慢性眼内压升高,通过该方法可以建立研究青光眼视网膜神经节细胞损害的模型。

AIM： To establish an experimental model of high intraocular pressure in mice by laser photocoagulation and to prepare for future research. METHODS： Experimental model of high intraocular pressure was induced unilaterally in 44 C57BL/6 mice. The fellow eye served as a control. TONO-PEN AVIA Tonomter was used to measure intraocular pressure （IOP） to guarantee IOP value at 1, 2, 4, 8wk. Slit-lamp biomicroscopy was performed throughout the period and the structural changes were assessed histologically. And then, their eyes were enucleated, postfixed, cryoprotected, and embedded in optimal cutting temperature medium. After hematoxylin and eosin stain （ HE stain ） , cryosections of the retina were observed under light microscope. TdT-mediated biotin-dUTP nick end labeling （ TUNEL ） was performed on the retinal sections to determine apoptosis rate. RESULTS： IOP of laser-treated eyes was significantly higher than that of control eyes from 1-8wk （P〈0. 05）. The highest IOP was 31mmHg, but only one eye. The IOP was mainly around 20mmHg. In laser-treated eyes, the angle of anterior chamber were narrow. Number of cells in the inner nuclear layer and retial gangllion cell layer was slightly lower than that in control eyes at 2wk, but by 4 and 8wk the number of cells was significantly lower than that in the control contralateral eyes. CONCLUSION： The laser photocoagulation of limbus causes chronic elevation of IOP and this method may be a promising experimental model for the investigation of biological mechanisms of glaucomatous retinal ganglion cell damage.