β-磷酸三钙复合骨髓间充质干细胞修复山羊骨软骨缺损的实验研究

β-TCP combined with BMSCs for repairing osteochondral defect of goats:a preliminary experiment study

目的将β-磷酸三钙(β-TCP)与山羊骨髓间充质干细胞(BMSCs)复合后,在生物反应器中分别向成软骨和成骨诱导,并植入骨软骨缺损处,观察软骨修复效果。方法分离、培养山羊BMSCs,在生物反应器中分别向成软骨及成骨诱导2周,植入骨软骨缺损部位。实验组分为A组:旋转力刺激+成软骨、成骨诱导组(力学刺激组),B组:单纯成软骨、成骨诱导组(无力学刺激组),并设空白对照组。术后12周和24周进行大体观察、组织学染色等,并行O'Driscoll Keeley and Salter评分。结果 A、B组均有新生软骨形成;A组软骨在12周与24周均优于B组(P<0.05);术后12、24周A组评分优于B组,差异有统计学意义(P<0.05)。对照组无新生软骨形成。结论将BMSCs复合于β-TCP,可用于组织工程修复骨软骨缺损;体外培养阶段的旋转力刺激有利于改善组织工程软骨的质量。

Objective To observe the effect of β-tricalcium phosphate （β-TCP） combined with BMSCs for repairing osteochondral defects of goats. Methods The goat BMSCs were isolated and cultured in vitro and inoculated into β-TCP, then the composites were induced towards osteoblasts and chondrocytes simultaneously in a double-chamber stirred- bioreactor for 2 weeks. The composites were implanted into the autologous osteochondral defect and were divided into 2 groups according to the mechanical stimulation in the bioreactor. Group A： induction respectively with mechanical stimulation, group B： induction respectively without mechanical stimulation group, non-repair group was control group. Gross observation, histological observation was performed after 12 weeks and 24 weeks. O＇Driscoll Keeley and Salter score was performed. Results Newborn cartilage were formed in group A and B, newborn cartilage tissue of group A was better than that of group B after 12 and 24 weeks. O＇Driscoll Keeley and Salter score showed that the quality of newborn cartilage of group A were better than that of group B after 12 and 2d weeks, and there were statistically significant difference （P 〈0.05）. Newborn cartilage hadn＇t formed in control group. Conclusion Osteochondral defect can be repaired by tissue engineering way of BMSCs as seed ceils and 13-TCP scaffold as scaffold, and mechanical stimulation in the bioreactor is helpful to improve cartilage quality.