生物工程骨在山羊脊柱横突间植骨融合的实验研究

Application of biological engineering bone fusion in spinal intertransverse bone of goat

目的构建一种理想的骨移植替代材料。方法以关中奶山羊为实验对象,体外分离并培养骨髓间充质干细胞(MSCs);构建人工骨复合物,以珊瑚羟基磷灰石(CHA)为支架,加入经过血管内皮生长因子(VEGF)基因转染的MSCs为三联复合物进行联合培养;将自体髂骨和构建好的生物工程骨植入山羊腰椎横突间进行对比观察研究。结果 8周触诊发现2组均无活动度。8周后摄X线片结果显示2组均已形成骨小梁。HE染色发现,基因增强工程骨在12周形成更加成熟的骨组织。生物力学测量显示2组移植骨在最大压缩强度和弹性模量上差异无统计学意义(P>0.05)。结论 CHAMSCs-VEGF三联成骨复合物在成骨的速度和质量上接近生物自体移植骨,有望广泛应用于临床。

Objective To construct a kind of ideal substitute material in bone transplantation. Methods In vitro mesenchymal stem cells （MSCs） derived from bone marrow of the adult Guanzhong dairy goats were cultivated. Artificial bone compound, which contained coralline hydroxyapatite （CHA） and MSCs and vascular endothelial growth factor （VEGF）, were constructed. Two different materials（CHA-MSCs-VEGF, autologous ilium） were implanted into transverse process gaps of each animal. Their ossification features were observed. Results The results of palpation showed that all groups had no activity after 8 weeks. The results of X-ray showed that all groups form bone trabecula after 8 weeks. HE staining revealed that artificial bone of CHA-MSCs-VEGF could form more mature bone tissue than autologous bone. The results of biomechanics showed that artificial bone of CHA-MSCs-VEGF had no difference with autologous bone on compressive strength and elastic modulus（P 〉0.05）. Conclusion Artificial bone of CHA-MSCs-VEGF is a kind of ideal succedaneum in bone graft.