小分子生物硫醇荧光探针研究进展

Recent Progress in Fluorescent Probes for the Detection of Biothiols

半胱氨酸(Cys)、同型半胱氨酸(Hcy)和还原型谷胱甘肽(GSH)等小分子生物硫醇在人的生理活动中起着重要作用.近年来,生物和环境样品中小分子生物硫醇的检测引起科学家们极大的兴趣,生物硫醇荧光探针和比色传感器得到快速发展.根据探针与生物硫醇的反应机理,包括利用小分子生物硫醇中巯基与探针反应、巯基和氨基协同与探针反应,综述两年来生物硫醇小分子荧光探针的设计、合成与应用进展.

Intracellular thiols, such as cysteine （Cys）, homocysteine （Hcy） and glutathione （GSH）, play crucial roles in maintaining biological systems. In last decade, developing small molecule probes for selective and sensitive detection of thiols under physiological conditions have attracted great interest. Fluorescent and colorimetric sensors are classified into two types of reactions according to their mechanism of reaction with thiols, including the reaction with mercapto group, the reaction with mercapto and amino group. Therefore, in this review we provide a general overview of the selected recent research involving the design and application of biological thiol-selective chemosensors.