铁棒锤氯仿部位的毒-效关系研究

Study on the Toxicity of Aconitum Pendulum Busch.

为探讨铁棒锤的毒效关系,提取分离其氯仿部位并采用HPLC分析其主成分,比较了该部位与铁棒锤其他活性部位的急性毒性,以热板法和扭体法考察了不同剂量铁棒锤氯仿部位对小鼠的镇痛作用.结果表明:铁棒锤不同部位对小鼠急性毒性强度差别较大,氯仿部位的毒性最强,正丁醇和石油醚部位次之,三者的LD50分别为37.514,6766.928,5492.337 mg/kg.不同剂量的铁棒锤氯仿部位均能提高小鼠热板痛阈值,呈剂量依赖性关系.20mg/kg铁棒锤60 min时的镇痛百分率为108.7%,远低于阳性对照10 mg/kg吗啡组(261.6%),但高于同剂量的铁棒锤总提物组(75.2%).不同剂量的铁棒锤氯仿部位均能有效抑制冰醋酸对所致小鼠扭体反应,呈剂量依赖性关系.20 mg/kg组的扭体抑制率为76.4%,明显高于200 mg/kg阿司匹林(50.5%)和20 mg/kg总提物组(51.0%).说明铁棒锤氯仿部位具有显著的毒性和镇痛活性呈剂量依赖性,推测其镇痛活性与毒性存在一定的关联.

To study the toxicity of Aconitum Pendulum Busch.its chloroform extract was separated and analyzed by HPLC . An acute toxicity test was used to compare the toxicity of different extracts , then hot-plate test and acid-induced writhing test were used to assess the analgesic effect of the 20, 10, and 5 mg/kg chloroform extracts.The results indicated that the toxicity varied among different extracts .The most toxic extract was that of chloroform , followed by n-butyl alcohol and petroleum ether, of which the LD50 was 37.514, 6766.928, 5492.337 mg/kg respectively.Different doses of chloroform extract could all increase the pain threshold in a dose-dependent manner in hot plate test .The analgesia percentage of the 20 mg/kg chloroform extract was 108.7%in 60 minutes, far less than that of the 10 mg/kg morphine （261.6%）, but was larger than that of the total extract （ 75.2%） .Different doses of chloroform extract could inhibit acetic acid induced writhing response in a dose-dependent manner .The writhing inhibition rate of the 20 mg/kg chloroform extract was 76.4%, significantly higher than that of the 200 mg/kg aspirin （50.5%） and the 20 mg/kg total extract （51.0%）.The chloroform extract exhibited significant toxicity and analgesic activity in a dose-dependent manner , and there is probably some correlation between the analgesic activity and toxicity .