P-糖蛋白与P53蛋白在乳腺癌中的表达及意义

The Expression of P-glycoprotein and P53 Protein in Breast Cancer and the Significance

目的探讨P糖蛋白(P-gp)和P53蛋白在乳腺癌中的表达及意义。方法 53例术前未进行化疗的乳腺癌标本经福尔马林固定、石蜡包埋后,用免疫组织化学方法检测乳腺癌组织中的多药耐药蛋白P-gp和P53的表达情况。结果 P-gp和P53在乳腺癌中的表达分别为49%(26/53)和51%(27/53)。P-gp表达与乳腺癌患者的年龄、组织学类型和淋巴结转移状况无关(P>0.05),而P53蛋白的表达除与年龄有关外(P<0.05),与组织学类型和淋巴结转移无关(P>0.05),P-gp在P53阳性病例中的表达明显高于P53阴性者(P<0.01)。结论免疫组织化学法检测P-gp、P53蛋白的表达对评估和指导乳腺癌化疗及预后具有一定的临床价值。

Objective To investigate the expression of P-glycoprotein （P-gp） and P53 protein in breast cancer and the significance. Methods Tumor samples from 53 patients with breast cancer, receiving no chemotherapy before surgery, were fixed in formalin and em-bedded with paraffin, and then the expression of P-gp and P53 protein was detected by immunohistochemistry. Results The expression of P-gp and P53 protein in breast cancer reached 49%（26/53） ad 51%（27/53） respectively;the expression of P-gp was not correlated with the age of the patients, histological types and lymph node metastasis （P〉0.05） while the expression of P53 protein was not correlat-ed with the histological types and lymph node metastasis （P〉0.05）but with the age of the patients （P〈0.05）;the expression of P-gp in P53 positive cases was much higher than that in P53 negative cases （P〈0.01）. Conclusions The detection of the expressions of P-gp and P53 protein by immunohistochemistry is of certain clinical value in evaluating the effect of chemotherapy for breast cancer and the prog-nosis.