SPAG9在子宫内膜癌中的表达及意义

The Expression of SPAG9 in Endometrial Cancer and the Significancein Significance

目的探讨睾丸癌(CT)抗原SPAG9在子宫内膜癌及子宫内膜癌HEC-1B细胞中的表达及意义,为临床诊断和治疗子宫内膜癌提供理论依据。方法免疫组化检测42例子宫内膜癌组织切片内SPAG9的表达。小干扰RNA转染HEC-1B细胞,实时定量PCR检测子宫内膜癌HEC-1B细胞SPAG9 mRAN表达;蛋白免疫印迹(WB)检测子宫内膜癌HEC-1B细胞中SPAG9和cyclin D1蛋白表达;CCK8检测SPAG9敲除后HEC-1B细胞增殖情况;流式细胞仪检测SPAG6敲出后HEC-1B细胞周期的变化。结果 42例肿瘤标本中83.3%(35/42)的标本SPAG9高表达。SPAG9在HEC-1B细胞中同样高表达。敲除SPAG9后,HEC-1B细胞G1期细胞明显增多,细胞周期蛋白cyclin D1表达降低。结论 SPAG9在子宫内膜癌及子宫内膜癌细胞中高表达;降低SPAG9的表达后可以影响cyclin-D1的表达,从而调控子宫内膜癌HEC-1B细胞的增殖。

Objective To study the expression of SPAG9, a recently found cancer testis （CT） antigens, in endometrial cancer and in the HEC-1B cells and the significance. Methods The expression of SPAG9 in tumor tissue or para-carcinoma tissue from 42 cases with endometrial cancer was evaluated by immune-histochemical staining;the HEC-1B cells were transfected with siRNA, the mRNA level of SPAG9 in HEC-1B was analyzed by quantitative real-time PCR, and the expressions of SPAG9 and cyclin D1 in HEC-1B were detect-ed by western belt （WB）;CCK8 assay was applied in detecting the proliferation of HEC-1B after the knockout of SPAG9 and flow cy-tometry in detecting the change of cell cycle. Results The expression of SPAG9 was high in the specimen from 35 cases out of the 42, the ratio reached 83.3%as well as in the HEC-1B cells;after the knockout of SPAG9, the cell proliferation was significantly reduced while the number of G1 cells was obviously increased. Conclusions The expression of SPAG9 in endometrial cancer and in cancer cells;the decrease of the expression of SPAG9 is of certain impact on the expression of cyclin-D1, which can regulate the proliferation of HEC-1B in endometrial cancer.