油茶花发育转录组测序及相关基因表达分析

Sequencing of Transcriptome Relevant to Flowering and Analysis of Floral-Related Genes Expression in Camellia oleifera

通过对油茶成花过程的转录组测序及其成花相关基因的表达分析,结果表明:转录组测序总共获得28 448 847个reads,5 742 023 480 bp数据量,GC含量为46.52%;拼接成大于200 bp以上的Unigenes有94 476条,N50长度为806 bp,其中1 kbp以上的Unigenes共12 643条,占Unigene总数的13.38%;Unigenes在各数据库中功能注释数目,在COG中有9 095条,在GO中有27 201条,在KEGG中有6 431条,在Swissprot中有24 534条,在TrEMBL中有36 393条,在Nr中有36 400条,在Nt中有30 858条;茎尖中FLC,FCA和FT基因表达量较AP1,AP2和PI基因低,FT基因(ID:Unigene60063)是油茶成花的关键基因,PI基因(ID:Unigene56059)与雄蕊发育关系紧密。

In this study,total RNA of stem apex and bud in Camellia oleifera was sequenced and its flowering relation genes were analysis. The results showed that transcriptome sequencing revealed a total of 28 448 847 reads, 5 742 023 480 bp data,and 46. 52% GC content in whole genome. The Unigenes numbers with more than 200 bp were 94 476,and the Unigenes were very similar with grape’s. The N50 length was 806 bp,and 1 kbp or longer Unigenes were 12 643,accounting for 13. 38% of total Unigenes database. The annotation number of Unigenes function in each database was ：9 095 Unigenes in COG,27 201 Unigenes in GO,6 431 Unigenes in KEGG,24 534 Unigenes in Swissprot,36 393 Unigenes in TrEMBL,36 400 Unigenes in Nr,and 30 858 Unigenes in Nt. The expression profile showed that FLC,FCA,FT expression were lower than AP1,AP2,PI expression in the stem apex. The FT gene （ ID：Unigene60063） may be a key gene for Camellia flower,and PI genes （ID：Unigene56059） are closely related with the stamen development.