摘要
目的:测定比合玛尔江散中没食子酸的含量。方法:采用高效液相色谱法,Pheomenex luna C18色谱柱(250×4.60mm,51μm),柱温35℃,流动相甲醇-0.2%磷酸(5:95)为流动相,检测波长273nm,流速1.0mL/min结果:没食子酸在21.88—218.80μg∥mL间呈良好的线性关系,r=0.9997,平均回收率为102.11%,RSD0.53%。结论:该方法具有操作简单,灵敏,准确的优点,分离效果显著,可用于余甘子的含量测定和本产品质量控制。
To determinate the contents of gallic acid in bihe maerjiang powder. Method :An HPLC method was used and Pheomenex luna C18 was selected (250×4.60mm,5μm), a column temperature was 35℃, methyl alcohol -0.2% Phosphoric acid (5:95 ) were mobile phase, the detecting wavelength and velocity were 273 nm and 1.0mL/min respectively. Results: There were established a good linear relation between 21.88 ~ 218.80 μg/mL of gallic acid, r = 0.9997, the average recovery methods had the advantages of simple and easy operation, effect. The methods were can be used to determination of was 101.00%, RSD was 0.86 %. Conclusion: The sensitive and accurate and exhibited a good separation contents and quality control of the fructus phyllanthi.
出处
《中国民族医药杂志》
2014年第8期26-27,共2页
Journal of Medicine and Pharmacy of Chinese Minorities