血清钙调神经磷酸酶活性与正常高值血压左室肥厚的研究

Research about the serum calcineurin activity and the perhypertension left ventricular hypertrophy

目的探讨血清钙调神经磷酸酶(CaN)在正常高值血压患者左室肥厚中的表达。方法共选取健康体检人群282例,根据血压分为正常血压组(n=78),正常高值血压组(n=122),高血压组(n=82);再将正常高值血压组根据左室质量指数(LVMI)和相对室壁厚度(RWT)值分为正常构型亚组(n=101)、左室肥厚亚组(n=21)。采用超声心动图测定计算LVMI、RWT,酶联免疫法测定血清CaN水平,比色法测定血清CaN活性,放射免疫法测定血清内皮素-1(ET-1),分析其与正常高值血压左室肥厚的关系。结果⑴在正常血压组、正常高值血压组、高血压组中,左室肥厚发生率分别为7.82%、17.21%、22.35%;LVMI、RWT、血清CaN活性、ET-1水平依次升高,差异具有显著性(P<0.05)。正常高值血压组、正常血压组两组之间的差异(P<0.05),比高血压组、正常高值血压组两组之间的差异(P<0.01)更具有显著性。⑵正常高值血压组中左室肥厚亚组血清CaN活性、ET-1均高于正常构型亚组(P<0.05)。结论正常高值血压左室肥厚发生率、CaN活性明显高于正常血压。活化的CaN促进了正常高值血压左室肥厚的发生发展。

Objective To investigate the role of the serum calcineurin （CaN）in perhypertension left ventricular hypertrophy. Methods From February 2011 to January 2014,282 healthy crowd were selected from The Jiangxi province People＇s Hospictal. Based on the blood pressure,all of them were divided into three groups：the normal blood pressure group （n=78）,the perhyperten-sion group （n=122） and the hypertension group （n=82）. Based on the left ventricular mass index （LVMI）and the relative ventricular wall thickness（RWT）,the perhypertension group were divided into two groups：the normal model subgroup（n=101） and the left ven-tricular hypertrophy subgroup （n=21）. LVMI,RWT were determined by echocardiography;serum calcineurin levels were measured by the Enzyme-linked immunosorbent assay method;serum calcineurin enzymatic activities were measured by the colorimetric and serum Endothelin-1（ET-1）levels were measured by the radioimmunoassay method. The role of the serum CaN,ET-1 in perhyper-tension left ventricular hypertrophy was analyzed. Results ⑴In the normal blood pressure group,perhypertension group,hyperten-sion group the incidence of the left ventricular hypertrophy were 7.82%,17.21%,22.35%respectively;the LVMI,RWT,serum CaN activity and serum ET-1 levels increased successively. There were significant differences （P〈0.05）. The differences between the normal blood pressure group and the perhypertension group（P〈0.05）were more significant than the differences between the perhy-pertension group and the hypertension group（P〈0.01）.⑵In the perhypertension group,the serum CaN activity、serum ET-1 levels in the Left ventricular hypertrophy subgroup were higher than the normal model subgroup （P〈0.05）. Conclusion The incidence and serum CaN activity of the left ventricular hypertrophy in the perhypertension group were higher than that of the normal blood pressure group. The CaN activated advanced the occurrence and development of the left ventricular hypertrophy in the perhyper-tension.