救必应水提物与抗菌药联用对产ESBLs细菌的抑制效果

Effects of Holly Bark water extractions combined with part of antibacterial agents on ESBLs-producing bacteria

【目的】探讨救必应水提取物与抗菌药联合诱导细菌传代的体外抑菌活性,为救必应的综合开发利用提供参考依据。【方法】采用96孔反应板二倍微量稀释法体外检测救必应水提取物与抗菌药的最小抑菌浓度(MIC),并以1/2 MIC的救必应水提取物与抗菌药联合诱导产超广谱β-内酰胺酶(Extended spectrum beta-lactamases,ESBLs)细菌传代,然后用微量棋盘稀释法测定救必应水提取物与抗菌药联合作用后的部分抑菌浓度指数(FICI)。【结果】救必应水提取物的MIC为1.0 g/mL,以0.5g/mL的救必应水提取物与阿莫西林、左氧氟沙星、克林沙星、磺胺间甲氧嘧啶、加替沙星、头孢噻呋钠6种抗菌药联合诱导产ESBLs细菌传代后MIC显著降低,联用后的FICI分别为0.375、0.750、0.750、0.515、0.750和0.750。【结论】救必应水提取物可明显增强抗菌药对产ESBLs细菌的抗菌活性,且呈协同或相加作用。

【Objective】This research was aimed to investigate the antibacterial activity in vitro induced by water extracts of the Holly Bark combined with antibacterial agents to provide references for the comprehensive development and utilization of Holly Bark. 【Method】The minimal inhibition concentration（MIC） of water extracts of Holly Bark and antibacterial agents were tested through twice micro-dilution method. Extended spectrum beta-lactamases（ESBLs）bacterial were induced and subcultured by the 1/2MIC of water extracts of Holly Bark combined with antibacterial agents. Fractional inhibitory concentration（FICI） indexes of extracts combined with antibacterial agents were determined by microdilution checkerboard techniques. 【Result】The MIC of water extracts of Holly bark was 1.0 g/mL. The MIC of ESBLs-producing bacterial decreased significantly after inducing by 0.5g/mL of water extracts of Holly Bark combined with 6 antibacterial agents, such as amoxicillin, levofloxacin, clinafloxacin, sulfamonomethoxine, gatifloxacin, ceftiofur sodium. FICI indexes were 0.375, 0.750, 0.750, 0.515, 0.750 and 0.750, respectively. 【Conclusion】Antibacterial activities of antibacterial agents against ESBLs-producing bacterial are enhanced significantly by water extracts of Holly Bark,showing additive effect or synergistic activity correlation.