As_2O_3/CD133抗体双面涂层药物洗脱支架的动物实验研究

Animal study of a double coated drug-eluting stent of As_2O_3 and CD133 antibody

目的:初步探讨As2O3/CD133抗体双面涂层药物洗脱支架在动物体内的治疗作用。方法:60只中华小型猪非高脂饮食喂养4周,随机分为球囊损伤组、假手术组和双面涂层支架组,造模后继续喂养4周,行冠状动脉光学相干断层成像(OCT)后处死动物,取心肌组织行免疫组化检测。结果:OCT图像显示,球囊损伤组冠状动脉斑块明显扩大,内膜增厚,纤维帽厚度明显增加;与球囊损伤组相比,双面涂层支架组斑块明显缩小,内膜增厚不明显,纤维帽厚度无明显增加;假手术组冠状动脉基本正常。双面涂层支架组冠状动脉的内膜/中膜面积比和厚度比低于球囊损伤组,但仍高于假手术组(P<0.05)。免疫组化结果表明,球囊损伤组心肌Bax和Bcl-22的表达明显高于假手术组,双面涂层支架组Bax和Bcl-22的表达明显低于球囊损伤组(P均<0.01),而与假手术组无明显差异。结论:As2O3/CD133抗体双面药物涂层支架能显著抑制冠状动脉内膜增殖,抑制心肌凋亡。

Objective：To investigate the effect of a double coated drug-eluting stent of As2O3 and CD133 antibody. Methods：A total of 60 China miniature pigs were feeded with non-fat diet for 4 weeks,then randomly divided into 3 groups：ballon injury group,sham group and stent implantation group.After relative treatment,pigs were raised for another 4 weeks.All animals were sacrificed after optical coherence tomography （OCT）examination and cardiac tissue was subjected to immunohistology analysis. Resuits：It was showed by OCT that coronary plaque enlarged,and endothelium and fibrous cap thickened in balloon injury group,but vascular lesions in stent implantation group were significantly reduced.The coronary intima / media thickness ratio and area ratio in stent implantation group was lower than those in ballon injury group,but higher than those in sham group （P 〈0.05 ）.The protein expression of Bax and Bcl-22 in ballon injury group was significantly higher than those in sham group and stent implantation group （P〈0.01）.But there were no significant differences between sham group and stent implantation group. Conciusion：As2 O3/CD133 antibody double coated drug-eluting stent significantly inhibits intimal proliferation and reduces myocardial apoptosis.