Salen-金属配合物与DNA中碱基的相互作用

Interaction of Salen-metal complexes with DNA base

参照文献方法,合成了N,N’-双(2-羟基-1-萘甲醛)缩邻苯二胺、乙二胺和1,3-丙二胺的3种Salen配体及其与Mn(Ⅲ)、Co(Ⅲ)形成的6种配合物,探究了它们对碱基的荧光猝灭效应.结果表明,对于同一碱基来说,Mn(Ⅲ)配合物比Co(Ⅲ)更易使碱基发生荧光猝灭;同种配合物与不同的碱基作用时,其猝灭强度大小顺序为胞嘧啶>腺嘌呤>鸟嘌呤,呈现出配合物对碱基作用的选择性;配合物的结构对荧光的猝灭也有影响.

Three kinds of Salen-Mn（Ⅲ）complexes [Mn（Ⅲ）Lx]CH3 COO and three kinds of Salen-Co （Ⅲ） complexes [Co （Ⅲ）Lx ]CH3 COO （L1 =N,N＇-bis （2 -Hydroxy -1 -naphthaldehyde ） o -phenylenediamine,L2 =N,N＇-bis （2-Hydroxy-1 -naphthaldehyde）ethylenediamine,L3 =N,N＇-bis （2-Hydroxy-1 -naphthaldehyde ） 1 ,3 -diaminopropane ） were synthesized.The results of their fluorescence quenching effect with DNA base show that Mn （Ⅲ）complexes is easier make fluorescence quenching than Co （Ⅲ）complexes with the same base.Quenching intensity is Cytosine〉Adenine〉Guanine while with the same complexes.The fluorescence quenching selectivity of complexes to bases was presented in the experiment,and the fluorescence quenching could be influenced by the structure of complexes as well.