摘要
植物溶血磷脂酸酰基转移酶(lysophospholipid acid actyltransferase,LPAT)是三酰甘油生物合成过程中的一个关键酶,在脂质的合成、种子的发育以及生物膜的流动性等方面有重要作用。本研究采用同源克隆的方法,获得LPAT4基因的2条全长CDS,长度分别为1143 bp和1140 bp。生物信息学分析表明,它们均具有LPLAT_LCLAT1样结构域,同属于LPLAT超基因家族,并分别被命名为BnLPAT4-1和BnLPAT4-2。时空表达分析表明,它们均为组成型表达基因,其中BnPAT4-1在叶中的表达量最高,而BnLPAT4-2在胚中的表达量最高。逆境分析表明,BnLPAT4-1和BnLPAT4-2在NaCl、PEG-4000、水渍、6BA和ABA的胁迫下呈现出不同的表达模式。极差分析显示,ABA对BnLPAT4-1的表达影响较大,而BnLPAT4-2的表达对PEG-4000更敏感。本试验为进一步研究油菜BnLPAT4基因功能奠定了基础。
Lysophospholipid acid actyltransferase (LPAT) is a pivotal enzyme of triacylglycerol biosynthesis and plays a key role in lipid synthesis, development of plant seeds and bio-membrane fluidity. Two LPAT4 full-length coding sequences (1143 bp and 1140 bp) were obtained by homology-based cloning, designated BnLPAT4-1 and BnLPAT4-2, respectively. Bioinformatics analysis revealed that they share the LPLAT_LCLAT1 like domain and belong to the LPLAT super family. Temporal and spatial expression results showed that BnLPAT4-1 and BnLPAT4-2 are genes of constitutive expression, with the highest expression in leaf and em-bryo, respectively. BnLPAT4-1 and BnLPAT4-2 showed different expression patterns under NaCl, PEG-4000, waterlogging, 6BA and ABA stresses. Pole difference analysis indicated that BnLPAT4-1 was regulated greatly by ABA, while BnLPAT4-2 was more sensitive to PEG-4000. These results are informative for function study of BnLPAT4 genes in Brassica napus.
出处
《作物学报》
CAS
CSCD
北大核心
2014年第10期1748-1755,共8页
Acta Agronomica Sinica
基金
国家高技术研究发展计划(863计划)项目(2012AA101107-3)
湖南农业大学作物学开发基金项目(ZWKF201303)资助
关键词
LPAT4
甘蓝型油菜
时空表达
非生物逆境
表达分析
LPAT4
Brassica Napus
Temporal and spatial expression
Abiotic stress
Expression analysis