118份甘蔗种质资源遗传多样性的AFLP分析

Genetic Diversity of 118 Sugarcane Germplasm Using AFLP Markers

采用AFLP标记技术,对来自中国、澳大利亚、美国、菲律宾、巴西和法国的118份甘蔗种质进行遗传多样性分析。采用10对引物组合共扩增出1310条谱带,其中多态性条带为1195条,多态性条带比率为91.2%。118份种质间的相似性系数在0.583~0.929之间,平均为0.750,多态信息量为0.2332,每个位点的有效等位基因数为1.3789。结果显示在相似性系数0.69处切割时,可划分为2个类群,第Ⅰ类群包括粤糖00-236、云蔗04-622和SP80-1816;第Ⅱ类群有115份种质,在相似性系数0.74处切割时,可将第Ⅱ类群划分为5个亚群。各国甘蔗种质亲缘关系较近,其中美国种质遗传多样性相对较丰富。

AFLP markers were used to assess the genetic diversity of 118 sugarcane germplasm from China, Australia, USA, Philippines, Brazil, and France. A total of 1310 bands were amplified with ten pairs of primers, 1195 of which were polymorphic bands, the percentage of polymorphic loci was 91.22%. The genetic similarity coefficient among 118 sugarcane germplasm ranged from 0.583 to 0.929 with an average of 0.750, the polymorphism information content was 0.2332, and the effective number of alleles per loci was 1.3789. The results showed 118 germplasm were divided into two groups at 0.69 of the similarity coefficient. The group I consisted of YT00-236, YZ04-622, and SP80-1816, which shared lower genetic similarity with the rest of 115 germ-plasm in another group. The 115 germplasm were divided into five sub-groups at 0.74 of the similarity coefficient. The sugarcane germplasm shared high genetic similarity among countries, and there existed much higher genetic diversity within germplasm introduced from USA.