HPLC测定心脑欣滴丸中红景天苷和酪醇含量

Determination of Salidroside and P-tyrosol in Xinnaoxin Dropping Pills by HPLC

目的建立HPLC同时测定心脑欣滴丸中红景天苷和酪醇含量的方法,为科学评价和有效控制心脑欣滴丸提供依据。方法采用WondasiL C18（4.6×250mm,5μm）色谱柱,以甲醇-水为流动相进行梯度洗脱,流速为1mL·min-1,检测波长275nm,柱温25℃。结果红景天苷、酪醇均能达到基线分离,线性范围分别为：25~400μg·mL-1（R2=1）,2.7~43.2μg·mL-1（R2=1）,平均回收率分别是99.82%（RSD=1.8%,n=6）,100.57%（RSD=2.5%,n=6）。结论该方法简单、准确度高、重复性好可用于测定心脑欣滴丸中红景天苷和酪醇的含量,可为该药的质量评价提供参考。

OBJECTIVE To develop a HPLC method for the determination of the content of Salidroside and P-tyrosol in Xinnaoxin Dropping Pills.METHODS The Wondasil C18 column （4.6 ×250mm,5μm） was used at 25℃with the linear gradient solvent system consisted of methanol-water.The detection wavelength was 275 nm and the flow rate was 1mL· min-1.RESULTS The linear range of Salidroside and P-tyrosol were 25~400μg· mL-1 （ R2 =1 ） ,2.7μg · mL-1 ~43.2μg · mL-1 （ R2 =1 ） , respectively;The average recoveries were 99.82%（ RSD=1.8%,n=6）,100.57%（RSD=2.5%,n=6）,respectively.CONCLUSION The method is simple,accurate and reproducible ,it can be used for determination of the content of Salidroside and P-tyrosol in Xinnaoxin Dropping Pills , which provids reference for quality evalution of the medicine.