摘要
目的建立了高效液相色谱法测定石淋通颗粒中夏佛塔苷含量的测定方法。方法采用DiamonsilTM C18色谱柱(5μm,4.6×250μg),甲醇-0.10%磷酸溶液(32∶68)为流动相,流速:1.0mL·min-1,检测波长272nm,柱温:35℃。结果夏佛塔苷进样量在0.006μg^1.044μg范围内与峰面积呈良好线性(r=0.9999),平均回收率为99.53%,RSD=1.05%(n=6)。结论该方法操作简便、准确、重复性好,可用于石淋通颗粒的质量控制。
OBJECTIVE To establish a HPLC method to determine the content of schaftoside in Shilintong Granules.METHODS The DiamonsilTM C18 （5μm,4.6 ×250mm） column was used.The mobile phase consisted of methanol-0.10%phosphoric acid （25∶75） at 1.0mL· min -1.The detection wavelength was 272nm and the column temperature was 35℃.RESULTS The calibration curve was in good linearity within 0.006 ~1.044μg （ r =0.9999 ）.The average recovery was 99.53%,RSD was 1.05%（ n=6 ）.CONCLUSION The method is simple ,ac-curate and reproducible.It can be used for content determination of schaftoside in Shilintong Granules.
出处
《海峡药学》
2014年第9期66-67,共2页
Strait Pharmaceutical Journal
