摘要
从取自某猪场表现初产母猪流产胎儿的肾脏、脾脏、肠系膜淋巴结组织,进行研磨后接种猪原代肾细胞,成功分离到一株病毒。该病毒的豚鼠红细胞血凝活性为27,用针对猪细小病毒结构蛋白VP2的特异性引物对分离病毒进行扩增,将扩增结果进行克隆测序,结果经BLAST分析后,证实分离到一株猪细小病毒。
A porcine parvovirus was identified using the porcine primary kidney cells with the mixture of the kidney, spleen, mesen-teric lymph node tissue from sows＆#39; abortion. The hemagglutination titer is 27 using the guinea-pig red blood cell. A target fragments towards the VP2 gene was amplified using PCR method, the sequenced results demonstrated that a porcine parvovirus was identified.
出处
《福建畜牧兽医》
2014年第5期8-9,共2页
Fujian Journal of Animal Husbandry and Veterinary medicine
关键词
猪细小病毒
分离
诊断
porcine parvovirus
isolation
diagnosis
