SPE-HPLC法测定益智果实中3种化合物的含量

Determination of three compounds in Alpinia oxyphylla by SPE-HPLC

目的：探索益智中不同类别指标性成分的含量测定方法,并测定不同种植基地圆柚酮、杨芽黄素和益智酮甲的含量。方法：采用固相萃取和高效液相色谱法测定益智果实中圆柚酮、杨芽黄素和益智酮甲的含量。色谱柱为菲罗门C6苯基柱（250×4.6mm,5μm）,以乙腈和水为流动相,流速1.0mL/min,柱温30℃,检测波长240nm。结果：该方法线性,精密度,稳定性均良好,圆柚酮、杨芽黄素和益智酮甲的平均回收率为98.61%-99.98%。不同种植基地中圆柚酮的含量比较接近,但是杨芽黄素和益智酮甲的含量差异较大。广西和广东产的益智中杨芽黄素含量较低,益智酮甲含量较高。海南产益智中杨芽黄素含量较高,益智酮甲含量较低。结论：该方法准确可靠,可用于益智药材及相关产品中圆柚酮、杨芽黄素和益智酮甲多指标成分的含量测定,为完善益智药材的质量标准提供参考。

Objective：To explore a characteristic chemical marker of Alpinia oxyphylla（A.oxyphylla）and to establish a method to determine the contents of nootkatone,tectochrysis and yakuchinone A in fruit of A.oxyphyllacollected from different sources.Method：The contents of nootkatone,tectochrysis and yakuchinone A were determined by SPE-HPLC.HPLC analysis was made on a C6-phenyl column（4.6mm ×250mm,5μm）with acetonitrile and water.The detection wavelength was 240 nm.Results：The HPLC method showed good linearity,precision and stability.The average recovery rates of three compounds were 98.61%to 99.98%.The content of nootkatone was similar at different sources.The contents of tectochrysis and yakuchinone A showed obvious difference among the plants collected from different sources.The content of yakuchinone A was high at Guangdong and Guangxi harvest zone and the content of tectochrysis was high at Hainan harvest zone.Conclusion：The method is accurate and reliable.It is appropriate for the quantitative determination of nootkatone,tectochrysin and yakuchinone A in A.oxyphylla and its production.