HCMV AD_(169)感染小鼠模型的建立

Establishment of mouse models infected with human cytomegalovirus strain AD169

目的探讨用HCMVAD169病毒株建立小鼠感染动物模型的可行性。方法 BALB/c近交系小鼠30只,随机分为三组,分别为病毒感染组、免疫抑制病毒感染组、正常对照组,病毒接种途径为腹腔注射。观察动物的变化,取各组小鼠全血分离有核细胞,用直接荧光免疫法检测CMVpp65早期抗原,以病理学方法检测动物组织器官的损伤特点。结果接种了病毒的二组小鼠都出现了明显体重不增、皮毛稀松、生长迟缓等病症,肺、肠、涎腺等组织器官发生了广泛病理损害,免疫抑制病毒感染组小鼠血液有核细胞内发现了pp65抗原阳性细胞。结论将HCMVAD169株接种BALB/c近交系小鼠建立感染模型是可行的,在机体免疫抑制状态HCMV可激活感染。

Objective To explore the feasibility of establishing mouse models infected with human cytomegalovims （HCMV） strain AD169. Methods Thirty BALB/c inbred strain mice were randomly divided into virus infection group, immune inhibi- tion and virus infection group, and normal control group. Mice were inoculated with HCMV strain AD169 by intraperitoneal in- jection. The characteristic manifestations of all mice were recorded each day. All mice＇s peripheral blood mononuclear cells werc separated, and then the early antigen of HCMV pp65 was detected using direct inmmnofluorescence. Pathological changes of all mice＇s major organs were detected by pathological methods. Results All the successfully established mouse models in virus in- fection group and immune inhibition and virus infection group demonstrated obvious unchanged body weight, growth retardation and fur rarefaction, and their organs and ti^ues, such as lung, intestine and salivary gland showed characteristic pathological damages, pp65 antigen lmsitive cells were found in nucleated cells of the mice in the immune inhibition and virus infection group. Conclusions It is feasible to establish validated BALB/c inbred strain mouse model infected with HCMV strain AD169. HC- MV can be actively infected in mice with immune inhibition.