流沙湾卵鳎微卫星标记的遗传多样性分析

Solea ovata Microsatellite Markers and Polymorphism Analysis

为分析湛江流沙湾海域优势渔种卵鳎的遗传多样性,应用微卫星标记技术,选用15对微卫星引物,以等位基因数、基因杂合度、多态信息含量、固定指数等遗传参数为指标,评估卵鳎群体内的遗传多态性。结果表明：共检测到90个等位基因,等位基因数从1-12不等,平均为6.0;有效等位基因数从1.0-8.4,平均为4.0,多态性位点比例为53%,显示其具有中等杂合子水平,其中8个多态位点的期望杂合度（He）为0.670-0.881,平均为0.800,观测杂合度（Ho）为0.353-1.000,平均为0.773,多态信息含量（PIC）值为0.616-0.870,平均为0.773,群体内固定指数F为-0.199-0.564,平均为0.046;流沙湾卵鳎群体具有高度遗传多样性。

Fifteen microsatellite markers were selected to analyze the genetic diversity of Solea ovata,one of the dominant species of marine fish in zhanjiang Liusha Bay. The following parameters werecalculated： number of alleles, gene heterozygosity, polymorphism information content, fixation indexand so on. Results are as the follows： 90 alleles were found; the nunber of alleles per locus ranged from1 to 12 with an average of 6.0; the number of effective alleles ranged from 1.0 to 8.4 with an average of4.0; the proportion of polymorphic loci is 53% which presents a medium level of heterozvgosity ,ofwhich 8 are polymorphic loci of expected heterozvgosity（He） ranged from 0.670 to 0.881 with anaverage of 0.800; the observed heterozygosity （Ho） ranged from 0.353 to 1.000 with an average of0.773; the polymorphic information content value （PIC） ranged from 0.616 to 0.870 with an average of0.773; the fixation index ranged from -0.199 to 0.564 with an average of 0.046, indicating that the S oleaovata groups of Liusha Bay area has a high degree of genetic diversity.