黄瓜离体子叶再生体系的建立

Regeneration System Establishment from Cotyledons in vitro of Cucumis sativus L.

以3份不同生态型黄瓜品种为试材,研究了外植体类型、激素配比和基因型对黄瓜离体再生能力的影响。结果表明:3种外植体材料形成愈伤组织的难易程度依次为子叶>下胚轴>根尖。3份不同生态型黄瓜品种均在M10(MS+1.5mg/L 6-BA+2.0mg/L AgNO3)培养基上表现出较高的不定芽再生诱导率,欧洲温室型黄瓜品种"优秀"为最佳基因型,在M10培养基上再生频率为14.71%,平均每外植体再生芽数为2.0,S2(MS+0.1mg/L 6-BA)培养基能明显促进不定芽的伸长,MS培养基添加0.15mg/L IBA可以使再生植株生根。

Using three different ecotype cucumber varieties as experiment materials,the effect of explant types,combination of hormones in the medium and genotypes on cucumber plantlet regeneration were studied.The results showed that the ability of differentiation buds was cotyledon〉hypocotyl〉root.In the medium of M10（MS＋1.5mg/L 6-BA＋2.0mg/L AgNO3）,three different ecotype cucumber varieties were shown a higher regeneration rate of adventitious buds.Among of the three varieties,european greenhouse cucumber＂Excellent＂had the highest shoot regeneration frequency which reached 14.71% and 2.0shoots per explant cultured in M10 medium.S2（MS＋0.1mg/L 6-BA）medium could significantly promote the elongation of adventitious buds,MS medium supplemented with 0.15mg/L IBA also could greatly make the regeneration of plant roots.