电磁辐射暴露对小鼠细胞免疫功能影响

Effect of electromagnetic field on cell immumity function in mice

目的观察平均功率密度90 mW/cm2电磁辐射暴露对细胞免疫功能影响。方法采用体内、体外淋巴细胞转化试验及迟发型超敏反应耳肿胀试验观察电磁辐射暴露对小鼠细胞免疫功能影响。结果平均功率密度90 mW/cm2电磁辐射暴露后第3、5、7天,脾脏淋巴细胞植物血凝素(PHA)刺激指数分别为(1.15±0.05)、(1.16±0.08)、(1.13±0.07),与对照组PHA刺激指数[分别为(1.09±0.03)、(1.12±0.11)、(1.21±0.13)]比较无明显变化;辐照后第3、5、7天,小鼠体内淋巴细胞转化率分别为(43±2.1)%、(53±3.0)%、(51±1.9)%,均明显高于对照组(32±3.6)%、(44±2.5)%、(41±2.4)%;辐照后第3、5天的小鼠耳肿胀指数分别为(2.67±0.37)、(2.93±0.52)mg,明显高于对照组,差异有统计学意义(P<0.05)。结论平均功率密度90 mW/cm2电磁辐射暴露后短期会导致小鼠细胞免疫功能亢进。

Objective To observe the effect of electromagnetic field（EMF） on cell immunity function.Methods After EMF exposure at the intensity of 90 mW /cm2 for 20 minutes,the lymphocyte transformation rate in vivo and in vitro and the ear blubber index of delayed type hypersensitivity test were determined on the day of 3rd,5th,and 7th.Results The phytohaemagglutinin stimulation indexes of the spleen lymphocyte in vitro were 1.15 ± 0.05,1.16 ± 0.08,and 1.13 ± 0.07,respectively,on 3rd,5th,and 7th day after the MEF exposure,without significant differences compared with those of the control（1.09 ± 0.03,1.12 ± 0.11,and 1.21 ± 0.13）,but the lymphocyte transformation rates in vivo were 43 ± 2.1 %,53 ± 3.0 %,and 51 ± 1.9 %,respectively,and were significantly higher than those of the control（P 〈0.05 for all）.On 3rd and 5th day after the EMF exposure,the ear blubber indexes were 2.67 ±0.37 mg and 2.93± 0.52 mg and significantly higher than those of the control（P 〈0.05 for all）.Conclusion Exposed to 90 mW /cm2 EMF could induce cellular immune hyperfunction in mice.