HPLC法同时测定呼伦贝尔产赤芍防治心血管系统疾病有效物质群中3种成分的含量

Simultaneous Determination of 3 Components in Effective substance Group of Paeonia lactiflora from Hulunbuir

目的：建立同时测定呼伦贝尔产赤芍防治心血管系统疾病有效物质群中芍药苷、羟基芍药苷、芍药内酯苷含量的方法。方法：采用高效液相色谱法。色谱柱为Inertsil ODS-SP（250 mm×4.6 mm,5μm）,流动相为乙腈-水溶液（梯度洗脱）,流速为1.0ml/min,检测波长为230 nm,进样量为20μl。结果：芍药苷、羟基芍药苷、芍药内酯苷的质量浓度分别在10.00-100.00、1.00-25.00、0.40-10.00μg/ml范围内与各自峰面积积分值呈良好的线性关系（r分别为0.999 5、0.9991、0.9992）;精密度、稳定性、重复性试验的RSD〈2%;平均加样回收率分别为98.17%（RSD=1.14%,n=6）、98.08%（RSD=1.50%,n=6）、98.07%（RSD=1.03%,n=6）。结论：该方法操作简便、快速,结果准确,可用于控制呼伦贝尔产赤芍的质量。

OBJECTIVE：To establish the method for simultaneous determination of peoniflorin,hydroxyl paeoniflorin and albiflorin in effective substance group of Paeonia lactiflora from Hulunbuir. METHODS：HPLC method was adopted. The determination was performed on Inertsil ODS-SP（250 mm×4.6 mm,5 μm）column with mobile phase consisted of acetonitrile-water（gradient elution）at the flow rate of 1.0 ml/min. The detection wavelength was set at 230 nm and sample size was 20 μl. RESULTS：The linear ranges of paeoniflorin,hydroxypaeoniflorin and albiflorin were 10.00-100.00 μg/ml（r=0.999 5）,1.00-25.00 μg/ml（r=0.999 1）and 0.40-10.00 μg/ml（r=0.999 2）,respectively. RSDs of precision,stability and reproducibility tests were all lower than2%. The average recoveries were 98.17%（RSD=1.14%,n=6）,98.08%（RSD=1.50%,n=6）,98.07%（RSD=1.03%,n=6）,respectively. CONCLUSIONS：The method is convenient,rapid and accurate,and can be used for quality control of P. lactiflora from Hulunbuir.