HPLC法同时测定旱莲草中木犀草素和芹菜素的含量

Simultaneous Determination of Luteolin and Apigenin in Eclipta prostrata by HPLC

目的：建立同时测定旱莲草提取物中木犀草素和芹菜素含量的方法。方法：采用高效液相色谱法。色谱柱为Nucleodur100-5 C18（250 mm×4.6 mm,5μm）,流动相为甲醇-0.2%磷酸水溶液（50∶50,V/V）,流速为1 ml/min,检测波长为350 nm,柱温为40℃结果：木犀草素和芹菜素的质量浓度均在0.02-50.00μg/ml范围内与各自峰面积积分值呈良好的线性关系（r分别为0.9995和0.9993）;精密度、稳定性、重复性试验的RSD〈2%;平均加样回收率分别为97.2%和98.9%,RSD分别为2.41%和2.91%（n均为6）。结论：该方法快速、简便,结果准确,可用于不同产地旱莲草药材的质量评价。

OBJECTIVE：To establish a method for simultaneous determination of luteolin and apigenin in Eclipta prostrata extract. METHODS：HPLC method was adopted. The separation was carried out on Neocleodur 100-5 C18（250 mm×4.6 mm,5 μm）column with mobile phase consisted of methanol-0.2% phosphoric acid（50∶50,V/V）at the flow rate of 1 ml/min. The detection wavelength was set at 350 nm and the column temperature was maintained at 40 ℃ RESULTS：The linear range were 0.25-50.00μg/ml for luteolin（r=0.999 5）and 0.25-50.00 μg/ml for apigenin（r=0.999 3）;RSDs of precision,stability and reproducibility tests were lower than 2%. Average recovery rates were 97.2%（RSD=2.41%,n=6）and 98.9%（RSD=2.91%,n=6）,respectively. CONCLUSIONS：The method is rapid,simple and accurate,and can be used for the quality evaluation of E. prostrata.