前列腺炎Ⅰ号对CNP大鼠前列腺组织TGF-β1、CTGF表达的影响

Effect of No.I decotion for prostatitis on the expressions of TGF-β1 and CTGF in prostatic tissue of rat models with chronic nonbacterial prostatitis

目的：探讨前列腺炎Ⅰ号治疗慢性非细菌性前列腺炎的作用机制。方法将70只雄性大鼠随机分为空白组（生理盐水10mlkg^-1）、模型组（生理盐水10mlkg^-1）、阳性组（前列舒乐颗粒2.4gkg^-1）和前列腺炎Ⅰ号小剂量组（10gkg^-1）、前列腺炎Ⅰ号中剂量组（20gkg^-1）、前列腺炎Ⅰ号大剂量组（40gkg^-1）。采用免疫性造模方法，空白组则注射生理盐水，30d后重复造模一次，45d后灌胃给药。给药28d后，剥取前列腺组织，免疫组化检测转化生长因子-β1（TGF-β1）和结缔组织生长因子（ CTGF）表达水平。结果与空白组相比，其余各组大鼠前列腺组织TGF-β1和CTGF表达水平明显升高，差异有统计学意义（P＜0.05）；与模型组相比，其余各组大鼠前列腺组织TGF-β1、CTGF表达水平明显降低，差异有统计学意义（P＜0.05）；与阳性组相比，其余各组大鼠前列腺组织TGF-β1、CTGF表达水平均有明显差异性，差异有统计学意义（P＜0.05）。结论前列腺炎Ⅰ号各剂量组能有效抑制CNP大鼠前列腺组织TGF-β1和CTGF的表达水平，改善前列腺组织的纤维化程度，其中，中、大剂量组效果更佳。

Objective To study the mechanism of No.I decotion in the treatment of CNP rats with chronic prostatitis. Methods Total of 70 rats were divided into 6 groups including normal contrast group （treated with normal saline 10mlkg-1）, model contrast group （treated with normal saline 10mlkg-1）, yang contrast group （treated wih qianlieshule 2.4g kg-1）, No.I decotion for prostatitis low-dose group （treated with10gkg-1）, middle-dose group （treated with 20gkg-1）, high-dose group （treated with 40gkg-1）. Rats in normal contrast group were injected subcutaneously with with 1 mL normal saline, and rats model in the other groups were done using self-immunitymodeling method, 30 days later the rats were retreated again,45 days later,they were administrated with drug mentioned above. 28 days later, prostate tissue of rats were resected for analysis of the amount of TGF-β1 and CTGF using immunohistochemical staining. Results that in The levels of TGF-β1 and CTGF in prostatic tissue of the rest groups were much higher than that in normal contrast group, the difference was significant（P〈0.05）. Compared to that in the model contrast group, the amount of TGF-β1 and CTGF in prostatic tissue of the rest groups were significantly increased（P 〈0.05）. The levels of TGF-β1 and CTGF in prostatic tissue of other groups were significantly changed （P 〈0.05） as compared with that in yang contrast group. Conclusion Every dose group of No.I decotion for prostatitis all effectively inhibited the expressions of TGF-β1 and CTGF in prostatic tissue of CNP rat models and alleviate the fibrosis severity of prostatic tissue, of which the middle and high dose groups were more effective.