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香蕉花提取物中抑制α-葡萄糖苷酶活性组分研究 被引量:5

Investigation of α-glucosidase Inhibitory Activity of Banana Flower Extract
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摘要 利用α-葡萄糖苷酶抑制剂阻止碳水化合物在体内的消化吸收,是治疗糖尿病的1种有效方式。采用体外α-葡萄糖苷酶抑制模型,以阿卡波糖为阳性对照,对香蕉花中不同极性组分进行活性评价。结果表明:各组分对α-葡萄糖苷酶均有一定抑制活性,其中石油醚部分对-葡萄糖苷酶的抑制作用最强,IC50达788.36 g/mL,低于对照阿卡波糖(IC50=999.31μg/mL),乙酸乙酯(IC50=1 877.77μg/mL)和正丁醇部分(IC50=2 117.78μg/mL)活性次之。该提取物最高活性部分对α-葡萄糖苷酶的抑制类型为竞争性抑制,根据Lineweaver-Burk方程求得Ki值为250.70μg/mL;对石油醚部分进行GC-MS分析,鉴定出29种化合物,主要化学成分为有机酸类(71.58%)、酯类(13.01%)、胺类(5.88%)、醛类(1.52%)、酮类(0.42%)化合物。 One effective way to treat diabetes is by suppressing carbohydrate digestion due to the utilization of α- glucosidase inhibitors. The inhibitory activities against a-glucosidase of different fraction from banana flower extract were as assayed in vitro and the results were compared with Acarbose as positive control. The results showed that different fractions of banana flower extract all had activity, and the petroleum ether extract had the highest inhibitory activity against α-glucosidase(IC50=788.36 Ixg/mL), more potent than the positive control acarbose(IC50=99.31μg/mL). The n-butanol (IC50=l 877.77 μg/mL) and ethyl acetate fraction of banana flower extract (IC50=2117.78 μg/mL) also showed high activity. A Lineweaver-Burk plot indicated that its inhibition of α-glueosidase was competitive, with a Ki value of 250.70 μg/mL. 29 ingredients were identified from the ethyl acetate fraction of banana flower extract by GC-MS. Organic acids (71.58%), esters (13.01%), amines (5.88%), aldehydes (1.52%) and ketanes (0.42%) were the main components.
出处 《中国食品学报》 EI CAS CSCD 北大核心 2014年第9期68-74,共7页 Journal of Chinese Institute Of Food Science and Technology
基金 国家自然科学基金项目(31201303) 海南省自然科学基金项目(309043)
关键词 香蕉花提取物 Α-葡萄糖苷酶 抑制活性 糖尿病 banana flower extract α-glucosidase inhibitory activity diabetes
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