柱前衍生高效液相色谱法检测8种枣水溶性多糖的单糖组成

Determination of Monosaccharide Components of Eight Varieties Jujube Water Soluble Polysaccharide by Pre-column Derivatization HPLC Method

建立了柱前衍生高效液相色谱法,对金丝小枣、大荔龙枣、晋枣、赞皇大枣、宣城圆枣、梨枣、无核小枣和骏枣中水溶性多糖的单糖组成及其比例进行分析比较。样品经清洗、烘干、粉碎、石油醚脱脂后,采用水提醇沉法得到水溶性多糖,经低温干燥后备用。水溶性多糖经三氟乙酸充分水解后,以1-苯基-3-甲基-5-吡唑啉酮（PMP）作为衍生剂,HPLC法分析枣水溶性多糖的单糖组成。结果表明：柱前衍生高效液相色谱法检测水溶性多糖的单糖组成衍生方法简单、线性关系好,检测灵敏度高,单糖的最低检出限为0.14~0.33 nmol。8种枣多糖均由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖6种单糖组成,其单糖组成比例存在很大差异。其中,甘露糖在各多糖中含量均较低,半乳糖醛酸含量普遍较高,葡萄糖除了在赞皇大枣和金丝小枣两种制干型枣中含量较低以外,在其余6个品种中含量较高。

A pre-column derivatization HPLC method was established for determination of Monosaccharide eomponents and their molar ratio of polysaccharides of eight varieties Ziziphus jujube. After cleaning, drying, crushing, petroleum ether degreasing, samples have been extracted by water and then alcohol precipitation. Water-soluble polysaccharide have been full hydrolyzed by trifluoroaeetic acid, using 1 - phenyl - 3 - methyl - 5 - pyrazolone （PMP） as derivatives, different monosaccharide components have been detected by HPLC. The results showed that column derivative HPLC determination by water soluble monosaecharide composition of polysaecharides derivative method is simple, good linearity and high detection sensitivity. The lowest detection limit of monosaceharide is 0.14 to 0.33 nmol. Eight kinds of jujube polysaccharide were composed of mannose, lee sugar, galacturonic acid, glucose, galaetose and arabinose, but there had big differences on its molar ratio Mannose content had the low level in all kinds of polysaccharide and galaeturonie acid content was generally high. Glucose had higher level in most of jujube varieties except Zanhuangdazao and Jinsixiaozao.