希金斯炭疽菌磷酸二酯酶生物信息学分析

Bioinformatics Analysis of Phosphodiesterases in Colletotrichum higginsianum

磷酸二酯酶(Pde)在植物病原菌G蛋白信号传导途径上发挥着重要作用。Pde已在粟酒裂殖酵母菌、稻瘟病菌等中有相关报道,然而,对于侵染十字花科植物的希金斯炭疽菌中Pde研究尚不深入。基于酿酒酵母中已经报道的典型Pde序列,利用Blastp以及关键词对炭疽菌蛋白质数据库进行比对、搜索,并通过SMART保守结构域分析。同时,通过对该菌中典型Pde氨基酸序列进行理化性质、疏水性、细胞信号肽、跨膜区结构、亚细胞定位以及二级结构等生物信息学分析,此外,通过对希金斯炭疽菌中的典型Pde与其他物种中的同源序列进行遗传关系比较分析。通过上述生物信息学分析,以期为深入开展Pde定位、功能研究等方面研究,同时,为进一步实现以控制病原菌G蛋白信号途径功能新的药物靶标的开发提供有力的理论支撑。

Phosphodiesterases（ Pde） as an enzyme plays important role in G protein signaling. Pde has been reported in Schizosaccharomyces pombe and Magnaporthe oryzae,however,it has not been reported in Colletotrichum higginsianum,which can infect many cruciferous plants. Based on the typical Pde sequence have been reported in Saccharomyces cerevisiae,to search Pde protein sequence from the protein databases of Colletotrichum spp. with the Blastp as well as the use of words,and to search the conserved domain in the SMART online. Meanwhile,bioinformatics analysis have made including the physicochemical properties,hydrophobic,signal peptide,trans-membrane domain structure,hydrophobic location and the secondary structure. In addition,analysis of genetic relationships through comparative Pde in C. higginsanum with homologous sequences of other species,in order to carry out the research including location and function of PDE,and provide strong theoretical foundation to further control pathogens for developing new drug targets with inhibiting G protein signaling pathways.