摘要
目的探讨Bcl-2抑制剂ABT737联合氯喹的抗肿瘤作用。方法选用人胶质瘤细胞U343细胞系,应用MTT方法检测ABT737和(或)氯喹对U343细胞活性的影响,western blot检测ABT737和(或)氯喹对U343细胞凋亡通路相关蛋白的作用。结果 ABT737显著降低细胞生存率,增加U343细胞凋亡相关蛋白Cleaved PARP、Cleaved Caspase-3及细胞色素C(cytochrome c)表达。当氯喹与ABT737联用时,二者联用与单用ABT737组相比,细胞活性显著降低,显著下调凋亡通路相关蛋白Cleaved PARP、Cleaved Caspase-3、细胞色素C表达。结论联用氯喹可提高人胶质瘤细胞U343细胞对Bcl-2抑制剂ABT737的敏感性。
Objective The purpose of this study is to investigate the effect of combination Bcl-2 inhibitor ABT737 with chloroquine.Methods Human glioma cell line U343 cells were chosen.The activity of U343 cells were determined by MTT.Apoptosis pathway-related proteins were determined by western blot.Results After U343 cells were treated with ABT737 for 24h,cell viability was significantly decreased in a dose-dependent manner.ABT737 concentration-dependently upregulated apoptosis-related protein （Cleaved PARP,Cleaved Caspase-3 and cytochrome C）.When combined with chloroquine,the cell viability of the combination group was significantly reduced,compared with that of ABT737 alone group.The apoptosis-related proteins of the combination group （Cleaved PARP,Cleaved Caspase-3 and cytochrome C）expression were also significantly reduced,compared with those of ABT737 alone group.Conclusion The combination of chloroquine can increase the sensitivity of human glioma cells U343 cells to Bcl-2 inhibitor ABT737.
出处
《中国实验诊断学》
2014年第9期1402-1405,共4页
Chinese Journal of Laboratory Diagnosis
