红景天对肺癌多药耐药细胞A549/DDP的促凋亡作用研究

Pro-apoptosis effects of rhodiola sacra on multidrug-resistant human lung carcinoma A549/DDP cells

目的观察红景天对人肺腺癌多药耐药细胞A549/DDP增殖和凋亡的影响,探讨其对化疗耐药的肺癌细胞的可能作用。方法以体外培养的人肺腺癌多药耐药细胞A549/DDP细胞作为研究对象,倒置显微镜观察红景天对肺癌细胞形态的影响;采用MTT法检测红景天对细胞生长的抑制作用;采用流式细胞仪检测细胞凋亡率;Western blot法检测凋亡相关蛋白cleaved caspase-3、cleaved PARP的表达。结果红景天能显著抑制A549/DDP增殖并促进其凋亡,上调cleaved caspase-3及cleaved PARP的蛋白表达量。结论红景天在体外对人肺腺癌多药耐药细胞A549/DDP细胞具有显著的抑制增殖和促进凋亡作用,其机制可能与其促进凋亡相关基因cleaved caspase-3与cleaved PARP的蛋白表达有关。

Objective To investigate the effect of rhodiola sacra on proliferation and apoptosis of human multidrug-resistant human lung carcinoma A549/DDP cells and its possible mechanisms of anti-tumor.Methods Cultured A549/DDP cells in vitro were used as the research object and their cell morphology were observed by inverted microscope. The inhibitory rate of cell proliferation was measured by MTT assay.The cell apoptosis was determined by flow cytometry.The protein expressions of cleaved caspase-3 and cleaved PARP were detected by Western blot analysis.Results Rhodiola sacra could inhibit the proliferation of A549/DDP cells and promote its apoptosis.The cleaved caspase-3 and cleaved PARP protein expression were increased in A549/DDP cells in a time-dependent manner induced by rhodiolasacra.Conclusion Rhodiola sacra can inhibit the proliferation and induce the apoptosis of A549/DDP cells,and the mech-anism may be associated with regulating protein expression of cleaved caspase-3 and cleaved PARP.