橙皮苷对牛牙根部牙本质体外再矿化的影响

Effect of hesperidin on remineralization process of bovine root dentine in vitro

目的探讨橙皮苷对牛牙根部牙本质再矿化的影响。方法取新鲜拔除的年轻牛恒牙26颗,高速硬组织切割机将牙根切成6 mm×6 mm×2 mm的牙本质切片,随机分为研究组、阳性对照组及空白对照组,每组6个切片。将各组标本浸入100 mL脱矿液中浸泡96 h,使牙本质形成脱矿病变。将研究组、阳性对照组及空白对照组切片分别放入5 000 mg/L橙皮苷、1 000 mg/L氟化钠及去离子水中浸泡2 h,各组切片放入再矿化液中8 h,连续循环8 d。收集整个循环过程中的再矿化液及各组牙本质切片,用羟脯氨酸法检测各组再矿化液中的羟脯氨酸含量,X线能谱分析法检测牙本质Ca、P含量。结果研究组、阳性对照组及空白对照组再矿化液羟脯氨酸含量分别为(22.80±11.78)、(16.43±7.87)、(64.16±3.92),研究组、阳性对照组再矿化液中羟脯氨酸含量均高于空白对照组(P<0.05)。研究组、阳性对照组牙本质Ca/P比例高于空白对照组,研究组低于阳性对照组(P均<0.05)。结论在体外条件下,橙皮苷具有抑制牛牙根部牙本质胶原降解、促进脱矿牙本质再矿化的作用。

Objective To investigate the effect of hesperidin on the remineralization process of bovine root dentine in vitro.Methods Twenty-six extracted young permanent bovine teeth were prepared into 6 mm ×6 mm×2 mm size of dentin slices, and randomly divided into3 groups：the research group, positive control group and blank control group , six slices in each group.Specimens were immersed in demineralizing solution for 96 hours, and then we formed dentin demineralization lesions.Specimens were incubated in testing solutions （5 000 mg/L hesperidin, 1 000 mg/L NaF and deionized water） for 2 hours and remineralizing solution for 8 hours, for 8 days.The remineralizing solution and dentin slices of each group were collected .Hydroxyproline content of the remineralizing solution was investigated by hydroxyproline assay , dentine Ca and P contents were analyzed by energy-dispersive X-ray spectrometry .Results Hydroxyproline contents of the research group, positive control group and blank control group in the remineralizing solution were respectively （22.80±11.78）, （16.43 ±7.87） and （64.16±3.92）.Hydroxyproline contents of the research group and the positive control group were higher than that of the blank control group （P〈0.05）.Dentine Ca/P ratios of the research group and the positive control group were higher than that of the blank control group , the research group was lower than the positive control group （all P〈0.05）.Conclusion Hesperidin inhibits dentine collagen degradation and enhanced remineralization in vitro.