大黄素对脓毒症急性肺损伤大鼠肺组织通透性的影响

Effects of emodin on lung permeability in acute lung injury in sepsis rats

目的观察大黄素对脓毒症急性肺损伤大鼠肺组织通透性的影响。方法成年雄性SD大鼠120只,随机分为空白对照组、假手术组、模型组及大黄素低、中、高剂量预处理组,每组各20只。采用盲肠结扎穿孔法复制脓毒症大鼠模型,假手术组探查取出盲肠后还纳腹腔。各组按取材时间不同又分为造模后3、6、12、24 h时点,每个时点5只大鼠。在不同时点处死大鼠,测量各组大鼠肺湿重与干重,血清与肺泡灌洗液蛋白浓度,计算干/湿比(W/D)与肺通透指数(LPI);HE染色观察肺组织的病理学变化,进行肺损伤评分。结果大黄素中、高剂量预处理组大鼠12、24 h的W/D值、LPI、肺损伤评分均低于模型组及大黄素低剂量预处理组(P均<0.01)。结论早期较大剂量预防性使用大黄素可有效减轻脓毒症大鼠肺水肿的程度。

Objective To investigate the effects of emodin on lung permeability in acute lung injury in sepsis rats . Methods A total of 120 adult male SD rats were randomly divided into 6 groups：blank control group , sham group , model group, low dose, medium dose and high dose emodin pretreatment groups , with 20 rats in each group.The rats with sepsis were induced by cecal ligation and puncture （CLP）.In the sham group, the abdominal cavity was closed after cecal exploration operation.According to the specimen collection time , each group was further divided into four time points：3 hours, 6 hours, 12 hours and 24 hours after modeling, with 5 rats in each time point.Rats were sacrificed at different time points to measure the lung wet/dry weigh ratio （ W/D） and protein concentration of the serum and bronchoalveolar lavage fluid and calculate the lung W/D and lung permeability index （ LPI） , to observe the pathological changes of lung tissue by using optical microscope and count lung injury score .Results The W/D, LPI, lung injury score in the medium and high dose emodin pretreatment groups were significantly lower than that of the model group and low dose emodin pretreatment group （all P〈0.01）.Conclusion Sepsis may cause pulmonary edema , which can be efficiently alleviated through early use of large doses of emodin .