防喘膏防治哮喘大鼠的疗效及其对血清IL-12、IL-13水平的影响

Effect of Fangchuangao on Bronchial Asthma and the Contents of IL-12 and IL-13 in Rats

目的观察防喘膏防治哮喘大鼠的疗效及其对血清白细胞介素(IL)-12、13水平的影响。方法将麻黄、大黄、白芥子、地龙、细辛、甘遂六味药按比例研成末,以生姜汁调和成膏状,敷贴于哮喘大鼠背部穴位;再以卵清白蛋白(OVA)激发哮喘,观察引发大鼠哮喘的潜伏期;末次雾化吸入卵清白蛋白后,采用ELISH法测定大鼠血清IL-12、IL-13水平。结果与哮喘模型组比较,防喘膏敷贴能延长哮喘大鼠的哮喘潜伏期(P<0.01);与正常对照组比较,哮喘模型组大鼠血清IL-12水平降低(P<0.01),IL-13水平升高(P<0.01);与哮喘模型组比较,防喘膏敷贴组及辅舒酮气雾剂组IL-12水平均升高(P<0.01),IL-13水平均降低(P<0.01);防喘膏敷贴组及辅舒酮气雾剂组IL-12、IL-13水平差异无统计学意义(P>0.05)。结论防喘膏穴位敷贴能延长哮喘大鼠的哮喘潜伏期,降低哮喘大鼠血清IL-13水平,提高IL-12水平可能是其作用机制之一。

To investigate the effect of Fangchuangao on bronchial asthma and the contents of IL-12 and IL-13 in rats. Methods Grinding ephedra, Chinese rhubarb, white mustard seed, earthworm,asarum and foli-um pyrrosiae were grinded into fine powder, and then were mixed with ginger juice to produce Fangchuangao. Fangchuangao was applied at points on the back of rats, and then chicken egg albumin（OVA） was used to stimu-late asthma. The incubation period of asthma was observed. After the last inhalation of OVA,the contents of IL-12 and IL-13 were determined by ELISA. Results Compared with model group, Fangchuangao application prolonged the incubation period of bronchial asthma. Compared with normal group, the serum level of IL-12 in model group was decreased and that of IL-13 was increased （P〈0.01）. Compared with model group, the serum level of IL-12 was increased and that of IL-13 was decreased in Fangchuangao group and Becotide group （P〈0.01）. The serum level of IL-12 and IL-13 was not significantly different between Fangchuangao group and Becotide group（P〉0.05）. Conclusion Fangchuangao can prolong the incubation period of bronchial asthma in rats by increasing the serum leve of IL-12 and decrease IL-13.