芪附汤对心肌缺血再灌注损伤大鼠心电图和血流动力学变化的影响

Effects of Qifu Decoction on Electrocardiogram and Hemodynamic Changes in Myocardial Ischemia Reperfusion Injury Rats

目的：观察芪附汤对大鼠心肌缺血再灌注损伤后不同时间点的心电图、血流动力学等相关指标的影响,探讨芪附汤对大鼠心肌缺血再灌注损伤的药效作用.方法：SD大鼠30只,随机分为5组：空白组、模型组、阳性组（盐酸维拉帕米片）4mg/kg、芪附汤高剂量组1.2g（生药）/kg、芪附汤低剂量组0.6g（生药）/kg,各组灌胃给药,连续5天,末次给药30min后,结扎大鼠心脏冠状动脉左前降支复制心肌缺血再灌注模型,Power-lab生理记录仪描记心电图、血流动力学曲线,观察造模后各个时间点心电图ST值和血流动力学相关指标的变化.结果：空白组与模型组比较,大鼠心肌缺血再灌注后10～30min,心电图ST段抬高值下降大于50％,表明造模成功.芪附汤高、低剂量给药组大鼠与模型组大鼠比较,造模后1h能显著性降低ST抬高值（P＜0.01）,造模后2h能显著升高大鼠左心室收缩压（LVSP）的降低值（P＜0.05）,造模后1h均能显著升高左室内压最大上升速率（＋ dp/dt max）的降低值（P＜0.01）,造模后2h均能显著降低左室内压最大下降速率（-dp/dt max）的抬高值（P＜0.05）,造模后2h和3h均能显著升高大鼠舒张压（DAP）的降低值（P＜0.05）;造模后2h,芪附汤高给药组大鼠能显著升高大鼠收缩压（SAP）、平均动脉压（MAP）的降低值（P＜0.05）.结论：芪附汤对大鼠心肌缺血再灌注损伤有显著的保护作用,并呈现一定的药效作用规律,为其临床治疗冠心病、心绞痛等提供实验依据.

Objective ： To observe the effect of Qifu Decoction on myocardial ischemia reperfusion injury in rats after different time points of electrocardiogram （ ECG）, blood flow dynamics and other related indicators, To study the effect of Qifu Decoction on myocardial is- chemia- reperfusion injury in rats. Methods： 30 SD rats were randomly divided into 5 groups：sham -operation group, model group, Verapamil Hydrochloride Tablets （4mg/kg）, the large dose groups of Qifu Decoction 1.2g（ crude drug）/kg, the low dose groups of Qifu Decoction 0.6g （ crude drug）/kg, Each group was ig administrted for 5 consecutive days. The model of myocardial ischemia reper- fusion injury in rats was induced by ligation of the left anterior descending of the coronary artery （LAD） after 30 minutes of end admin- istration. Electrocardiogram （ECG）, Hemodynamic curve was recorded by The Power- lab physiological grapher, Each time point ST value changes of ECG were observed and hemodynamic indexes. Result： Compared the blank group and the model group, After myo- cardial ischemia - reperfusion 10 to 30 min in rats,The increase values of Ecg ST segment drop more than 50% ,That building success. Qifu Decoction of high and low doses in rats compared with model group rats, building 1 h after ST elevation values have dropped sig- nificantly （ P 〈 O. 01 ）, 2 h after building can marked increase in rats with left ventricular systolic pressure （LVSP） （ P 〈 0.05 ）, lower value of building 1 h after all can the marked increase in left ventricular isovolumetric systolic interior maximum pressure rise rate （ ＋ dp/dt Max） of lower value （P 〈 0. O1 ）, 2 h after building can significantly reduce the left ventricular isovolumetric diastolic pressure reduced rate of the largest indoor （ ＋ dp/dt Max） of higher value （P 〈 0.05）, 2 h and 3 h after building are to a significant rise in di- astolic blood pressure of rats （DAP） of lower value （P 〈 0.05 ） ; Building after 2 h, stilbene decoction high dose group of rats signifi- cantly elevated systolic blood pressure （SAP） in rats, reduce the value of the mean arterial pressure （MAP） （ P 〈 0.05）. Conclusion ： Qifu decoction has a obviousprotective effect on myocardial ischemia -reperfusion injury. And Render certain therapeutic effects rule, so provide the experimental evidence its clinical treatment of coronary disease, angina pectoris etc.