加速溶剂萃取-超高效液相色谱(UPLC)测定土壤中苯并(a)芘

Determination of BaP in Soil by Accelerated Solvent Extraction and Ultra High Performance Liquid Chromatography

本文优化了加速溶剂萃取-超高效液相色谱测定土壤中苯并（a）芘的方法。样品经加速溶剂提取，逐级减压浓缩，0.25um滤膜过滤净化，超高效液相色谱（UPLC）测定。结果表明，标准溶液苯并（a）芘含量在3.125～lOOug／L范围内，苯并（a）芘的线性呈良好的线性关系，相关系数为0.9999，该方法的检出限和测定下限分别为0.015μg/kg和0.060μg/kg。将该方法用于4个地区土壤样品的测定，苯并（a）芘含量在0.04～6.26ug／kg之间，空白加标回收率为75.2％～96.4％之间，各项指控指标符合检测要求。

Analytical method based on accelerated solvent extraction-ultra high performance li （ UPLC ） for determination the concentration of Benzo （a） pyrene （ BaP ） in soil was optimized qui in d chromatography this study. The analysis procedure commenced with accelerated solvent extraction （ASE）, gradual condensation in vacuum and followed by 0.22 um clean-up. The results showed that when the standard BaP concentration was between 3.125～ 100. 0ug/L, the BaP content showed a fine liner relationship, and the correlation coefficient was 0.9999. The limit of detection （LOD） and the limit of quantification （LOQ） in this method were 0.015μg/kg and 0.060 μg/kg,respectively. The method was applied in the range of 0.04 ～ 6.26ug/kg,and the recoveries of BaP blank standard ranged from 75.2%～96.4%,which all fell within the acceptable range set by the QC system.