摘要
受体制备是精原干细胞(spermatogonial stem cells,SSCs)成功移植的基础,高效、安全的受体制备方法对研究精子发生机制、雄性不育及濒危动物保护等具有重要的理论和实际意义。目前已建立了白消安腹腔(阴囊、睾丸)注射法、辐射法、热激处理法等受体制备方法,并均已取得一定进展,但不同制备方法均存在一定局限性,影响移植效率;同时不同受体制备方法、不同实验SSCs移植时机不同,移植效果也不尽相同。本文对近年的受体制备方法和不同实验移植时间的确定进行了简要概述,以期为通过优化这两个环节提高精原干细胞移植效率提供借鉴。
The recipient preparation is the basis for transplantation of spermatogonial stem cells(SSCs). The high efficient and safe technology of recipient preparation for SSCs transplantation has theoretical and practical significance in the study of spermatogenesis mechanism, treatment of male sterility and protection of the endangered animals. At present, some methods for recipient preparation have been setup such as intraperitoneal(scrotum, testes) busulfan injection, testicular irradiation, heat shock treatment, but the limitation of different method affects the efficiency of SSCs transplantation; At the same time, the different methods of recipient preparation and transplantation time can affect the efficiency too. In this review, we reviewed the study on recipient preparation and the selection of transplantation time, we hope to provide some references for optimizing the recipient preparation and transplantation time to improve the efficiency of SSCs transplantation.
出处
《中国畜牧杂志》
CAS
北大核心
2014年第19期91-95,共5页
Chinese Journal of Animal Science
基金
国家"十二五"科技支撑计划(2011BAD19B02)
奶牛产业技术体系北京创新团队项目
国家自然基金项目(31372296)
中国农业科学院北京畜牧兽医研究所科技创新团队项目(cxgc-ias-05)
关键词
精原干细胞
受体制备
移植时机
spermatogonial stem cells
recipient preparation
transplantation time