ECRG4在甲状腺乳头状癌组织中表达水平的研究

Expression levels of ECRG4 gene in thyroid papillary carcinoma tissues

目的研究甲状腺乳头状癌组织中ECRG4基因的表达水平,及ECRG4在甲状腺乳头状癌中的作用。方法收取甲状腺乳头状癌组织30例作为病例组,选取相对应的20例正常甲状腺组织作为对照组,通过实时荧光定量PCR法(QRT-PCR)以及免疫组织化学法,检测ECRG4基因及其蛋白在甲状腺组织中的表达情况。结果 QRT-PCR检测结果显示,ECRG4 m RNA在甲状腺乳头状癌中呈现高表达,其表达水平明显高于对照组(P<0.01);免疫组织化学结果也显示,甲状腺乳头状癌中ECRG4蛋白呈现出高表达,显著高于对照组(P<0.01)。结论甲状腺乳头状癌组织中ECRG4基因及其蛋白呈现高表达,ECRG4在甲状腺乳头状癌中可能为一个促癌基因,其在甲状腺癌的发生及发展过程中起着重要作用。

Objective To study expression levels of ECRG4 gene in thyroid papillary carcinoma tissues and explore the role in thyroid papillary carcinoma. Methods The thyroid papillary carcinoma tissues of 30 cases were collected and taken as the case group, normal thyroid tissues of 20 cases were selected as control group. QRT- PCR and immune histochemical methods were adopted to detect the ECRG4 gene expression levels in the thyroid tissues. Results The results of QRT- PCR detection showed that the ECRG4 expression levels were up-regulated in thyroid papillary carcinoma tissues, which was significantly higher than that of the control group （P〈 0.01）;immunohistochemical results also confirmed that the ECRG4 protein expression level in thyroid papillary carcinoma tissues were enhanced significantly than that of the control group （P〈 0.01）. Conclusion The expression levels of ECRG4 gene in hyroid papillary carcinoma tissues were up-regulated, so ECRG4 might be an oncogene in thyroid papillary carcinoma, it maybe play an important role in tumor occurrence and development.