高强度运动训练对大鼠骨骼肌细胞IL-6表达的影响

Effect of high intensive exercise training on IL-6 expression in skeletal muscle cells of rats

目的：研究高强度运动训练条件对大鼠骨骼肌细胞IL-6表达水平的影响。方法首先建立高强度运动训练大鼠动物模型，然后采用双抗体夹心ELISA法，检测高强度运动训练后大鼠外周血以及骨骼肌细胞IL-6的表达水平。结果经高强度运动训练后，大鼠骨骼肌细胞及外周血IL-6含量均有所升高。结果显示；经过高强度运动后，大鼠血清中IL-6的含量与对照组相比有明显升高，各组结果差异有统计学意义，P〈0.01，高强度运动组大鼠骨骼肌细胞中IL-6的含量与对照组相比，结果差异有统计学意义，P〈0.05。但是一次性力竭运动组大鼠外周血及骨骼肌细胞中IL-6的含量与对照组相比，结果差异无统计学意义，P＆gt;0.05。结论高强度运动训练可以诱导大鼠骨骼肌细胞及外周血中IL-6的表达，发挥抗炎作用。

Objective To investigate the effect of high intensive exercise training on IL-6 expression in skeletal muscle cells of rats. Methods Double-antibody sandwich ELISA was used to measure the level of IL-6 in between the experiment group （high intensive exercise training） and the control group. Results The level of IL-6 in serum and skeletal muscle cells of rats increased when the experiment group was trained for high intensive exercise. The results showed that the content of IL-6 in serum of the experiment group was significantly higher than that of control group ,P〈0.01. Meanwhile, the content of IL-6 in skeletal muscle cells of the experiment group was increased obviously in comparing with the control group ,P〈0.05. However, the content of IL-6 reflected no significant differences between one bout exhaust exercise group and control group , P＆gt;0.05. Conclusion High intensive exercise training may promote the expression of IL-6 in serum and skeletal muscle cells of rats, which would be beneficial for treating inflammation.