猪卵丘包裹层数与猪卵母细胞质量的相关性研究

Relevance Analysis between the Layers of Cumulus Cells and the Quality of Porcine Oocyte

为探讨如何从大量的未成熟卵母细胞中挑选出合适的卵母细胞用于体外成熟培养,本研究根据猪卵丘-卵母细胞复合体（COCs）的颗粒细胞层数和胞质情况,把挑选的COCs分成A、B、C3个等级;体外成熟培养42~44 h后,所获得的成熟卵母细胞做孤雌激活,比较孤雌胚的发育能力。结果表明：A、B、C3级卵母细胞的成熟率之间没有差异（P〉0.05）,但是A级卵母细胞与B级卵母细胞的孤雌胚胎的囊胚率显著高于C级卵母细胞（P〈0.05）。说明卵丘细胞包裹层数不会影响卵母细胞的成熟率,但会影响其潜在的发育能力。

In order to choose appropriate oocytes from vast immature oocytes for in vitro maturation culture,based on granular cell layers of pig cumulus oocyte complexes（COCs） and the cytoplasm, the selected COCs is divided into A, B, C grades. After in vitro maturation culture for 42-44 h, parthenogenetic activationwere made to get matured oocytes, then compare the embryos viability. Results showed that there was no difference between oocyte maturation rate（P〉0.05）, but A oocytes and B oocytes parthenogenetic embryo blastocyst rate is significantly higher than grade C oocytes（P〈0.05）. This indicated that the layers of cumulus cells did not affect the maturation rate, but will affect the development of the embryos potential ability.