HPLC法测定冰樟桉氟轻松贴膏中醋酸氟轻松含量

Determination of fluocinolone Acetonide in Borreol Camphor Euclyptus Oil and Fluocinonide Patches by HPLC

目的：建立HPLC法测定冰樟桉氟轻松贴膏中醋酸氟轻松的含量。方法：采用Diamonsil C18（150mm×4.6mm,5μm）色谱柱,流动相：甲醇-乙腈-水（60∶10∶30）,流速：1.0mL·min-1,检测波长：240nm,柱温：30℃。结果：醋酸氟轻松在3.19~63.76μg·mL-1范围内线性关系良好（r=0.9999）,平均回收率为96.8%（n=9）,RSD为2.0%。结论：该方法专属、准确,可用于冰樟桉氟轻松贴膏中醋酸氟轻松的含量测定。

Objective： To establish an HPLC method for determination of fluocinolone acetonide in borreol camphor euclyptus oil and fluocinonide patches. Methods： Diamonsil C18 （150mm × 4.6mm 5gin）column was used. The mobile phase was methol-acetoni- trile-water （60 ： 10 ： 30） with a flow rate of 1.0 mL · min-1. The detection wavelength was set at 240nm. The column temperature was 30 ℃. Results：The calibration curve of fluocinolone acetonide showed a good linearity at 3.19 ~ 63.76 μg ·mL^-1 （r=0.9999 ）, and the average recovery was 96.8 % （n= 9）, RSD= 2.0 %. Conclusion：This method is specific and accurate for determination of fluocinolone acetonide in borreol camphor euclyptus oil and fluocinonide patches.