虎杖根茎超临界CO_2萃取物GC/MS分析及抗细胞迁移活性评价

GC/MS Analysis and Anti-Migration Activity of the Supercritical Fluid Carbon Dioxide Extracts from the Rhizomes of Polygonum cuspidatum

为了研究虎杖根茎的挥发性化学成分及其对人肝癌细胞Huh-7的细胞迁移活性,采用超临界CO2萃取和GC/MS方法分析鉴定虎杖根茎中的低极性化合物,并利用体外伤口愈合模型评价其抗细胞迁移活性。结果表明:从虎杖根茎超临界CO2萃取物中分离鉴定了33个化合物,主要成分为莪术烯、丙二醇、醋酸、吉玛烯B、2,3-丁二醇等,质量百分比分别为41.8%、12.2%、6.9%、6.5%和5.9%;体外伤口愈合模型测试表明,虎杖根茎超临界萃取物在浓度为10mg/L,作用24h,能抑制人肝癌细胞Huh-7的细胞迁移活性,抑制率为19.85%。本实验可为阐明虎杖药效的物质基础提供参考。

To investigate the volatile components from the rhizomes of Polygonum cuspidaturn and its anti-migration activity, the rhizomes of Polygonum cuspidatum was extracted with supercritical fluid carbon dioxide extraction （SFE-CO2） and analyzed by gas chromatography-mass spectrometry （GC/MS）. The would healing model was used to evaluate the effects of the supercritical extracts on human hepatoma Huh-7 cell migration. Thirty-three compounds are identified from the SFE-CO2 extracts of the rhizomes of Polygonum cuspidatum, the major constituents are curzerene （41.8%）, propylene glycol （12.2%）, aceticacid （6.9%）, germacrene B （6.5%） and 2,3-butanediol （5.9%） ect. The in-vitro would healing model assay shows the volatile constituents of the rhizomes of Polygonum cuspidatum possess effective anti-migration activity against human hepatoma Huh-7 cells by 19.85%. The results will provide foundations for understanding the constituents and further exploritatin of Polygonurn cuspidatum.